In this first comprehensive resource on the topic, one of the world′s most distinguished single molecule scientists has put together a team of pioneers in the field to show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
Indispensable reading for a growing number of scientists.
Single Molecule Study for Elucidating the Mechanism used by Biosystems to Utilize Thermal Fluctuations
Imaging and Molecular Motors
Signal Transduction across the Plasma Membrane
Dynamics of Membrane Receptors: Single Molecule Tracking of Quantum Dot Liganded Epidermal Growth Factor
Studying Dynamics of Ligand–Receptor Complexes by Single–Molecule Techniques
RNA in Cells
Protein Dynamics and Interactions
Two Rotary Motors of ATP Synthase
Single Molecule FRET Studies of Helicases and Holliday Junctions
High–speed Atomic Force Microscopy for Nano–visualization of Biomolecular Processes
Force–clamp Spectroscopy of Single Proteins