Single Molecule Dynamics in Life Science

  • ID: 2179646
  • Book
  • 346 Pages
  • John Wiley and Sons Ltd
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Advances in instrumentation over the last few years have established single molecule measurements as a major method in many biological laboratories. Exploiting the full power of such measurements is dependent on a good understanding of single molecule behavior and enzymology.

In this first comprehensive resource on the topic, one of the world′s most distinguished single molecule scientists has put together a team of pioneers in the field to show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Indispensable reading for a growing number of scientists.
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A Road Map to Single Molecule Dynamics

Single Molecule Study for Elucidating the Mechanism used by Biosystems to Utilize Thermal Fluctuations

Imaging and Molecular Motors

Ion Channel

Signal Transduction across the Plasma Membrane

Dynamics of Membrane Receptors: Single Molecule Tracking of Quantum Dot Liganded Epidermal Growth Factor

Studying Dynamics of Ligand–Receptor Complexes by Single–Molecule Techniques

RNA in Cells

Protein Dynamics and Interactions

Two Rotary Motors of ATP Synthase

Single Molecule FRET Studies of Helicases and Holliday Junctions

High–speed Atomic Force Microscopy for Nano–visualization of Biomolecular Processes

Force–clamp Spectroscopy of Single Proteins
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"An invaluable resource to rapidly familiarize oneself with the diverse approaches to single molecule experimentation." (The Quarterly Review of Biology, December 2009)
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