This first overview of the topic opens with a general introduction to the use of mass spectrometry in pharmaceutical screening, followed by a detailed description of recently developed analytical systems for use in the pharmaceutical laboratory.
Applications range from simple binding assays to complex screens of biological activity and systems containing multiple targets or ligands –– all highly relevant techniques in the early stages in drug discovery, from target characterization to hit and lead finding.
A ready reference for medicinal and organic chemists, spectroscopists, and those working in the pharmaceutical industry.
Methods, Principles and Instrumentation
Drug Screening Using Gel Permeation Chromatography Spin Columns Coupled with ESI–MS
ALIS: An Affinity Selection–Mass Spectrometry System for the Discovery and Characterization of Protein–Ligand Interactions
Library Screening Using Ultrafiltration and Mass Spectrometry
Continuous–Flow Systems for Ligand Binding and Enzyme Inhibition Assays Based on Mass Spectrometry
Frontal Affinity Chromatography –
Mass Spectrometry for Ligand Discovery and Characterization
MS–Binding Assays – an Alternative to Radioligand Binding
Laser Desorption Assays –
MALDI–MS, DIOS–MS, and SAMDI–MS
Tethering: Fragment–Based Drug Discovery by Mass Spectrometry
Interrogation of Noncovalent Complexes by ESI–MS: A Powerful Platform for High Throughput Drug Discovery
Quantification of Protein–Ligand Interactions in Solution by H/D Exchange (PLIMSTEX)
Protein–Targeting Drug Discovery Guided by Hydrogen/Deuterium Exchange Mass Spectrometry (DXMS)
Mass Spectrometry in Early Pharmacokinetic Investigations