This edition retains the successful features of its predecessor:
- Clear explanation of all steps in the analytical procedure, starting with the choice of a suitable TLC technique and finishing with data evaluation and documentation.
- Special emphasis on the correct choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering precoated layers, solvents and developing chambers, including information on suppliers, among others.
- Many practical hints for troubleshooting.
- Detailed description of using TLC in compliance with GLP/GMP regulations, including the required documentation , allowing readers to easily compile their own standard operating procedures.
- Numerous color illustrations.
From the reviews of the first edition:
"A clear, step–by–step practical guide to TLC, covering every stage of the process from plate selection and sample application to development and quantification. At every stage potential pitfalls are clearly signposted and each chapter contains a profusion of pracitcal tips. Even a seasoned TLC practitioner should find something of use or interest in this book which complements most existing texts."The Analyst
What is TLC?
When is TLC used?
Where is TLC used
How is the result of a TLC represented?
What kinds of reference substances are used in TLC?
The literature on TLC
Why use precoated layers?
How are precoated layers produced?
What kinds of precoated layers are there?
What are precoated layers used for?
Criteria for choosing stationary phases in TLC
Effect of the stationary phase when the solvents are identical
Recommendations for the procurement and storage of precoated layers
Problems in the nomenclature and classification of precoated layers
BEFORE TLC DEVELOPMENT
Handling of the precoated layers
Application of the sample
Positioning of the sample
Drying before development
SOLVENT, DEVELOPING CHAMBERS AND DEVELOPMENT
TLC developing chambers
Development of thin–layer chromatograms
Drying after development
EVALUATION WITHOUT DERIVATIZATION
Visual direct evaluation
Optical direct evaluation using special equipment
Coupling methods for substance identification
Documentation without or before derivatization
Irradiation with high energy light
Reaction with reagents
Special types of derivatization
Treatment of derivatized chromatograms
EVALUATION AFTER DERIVATIZATION
Evaluation by TLC scanner
Evaluation by video system
Evaluation by flat–bed scanner
Description of a thin–layer chromatogram
Documentation by reproduction, tracing or photocopying
TLC scanner documentation
Flat–bed scanner documentation
GMP/GLP–CONFORMING TLC WORK
Validation of TLC methods
Use of qualified
GMP/GLP–conforming rawdata sheets
Examples of GMP/GLP–conforming testing procedures
Definite loading of a substance
TLC of sensitive substances
SPECIAL METHODS IN TLC
Automated Multiple Development
Overpressured Layer Chromatography
High Pressure Planar Liquid Chromatography
Combination of TLC with Flame Ionization Detector or Flame Thermoionic Ionization Detector
Rotation Planar Chromatography
Chromatography and art