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Probes and Tags to Study Biomolecular Function. for Proteins, RNA, and Membranes

  • ID: 2179714
  • Book
  • 193 Pages
  • John Wiley and Sons Ltd
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In order to study living cells and their component molecules, two basic strategies have emerged in recent years. The first is to apply a soluble marker molecule, a ′probe′, that partitions to a particular cellular domain, and which can subsequently be measured. The second is to chemically or genetically modify a molecule of interest with a built–in probe, which is then called a ′tag′.

This handy reference systematically reviews current experimental methods and enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step–by–step protocols, illustrated by typical research applications. Commencing with probing the lipid bilayer, the text then moves on to discuss probing proteins –– including membrane proteins –– and nucleic acids. This is the first single publication to incorporate chemical markers, fluorescent probes and genetic tags to allow a well–informed comparison of different solutions for similar problems in molecular analytics.
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Fluorescent Sterols to Study Cholesterol Trafficking in Living Cells

Lipid Binding Proteins to Study Localization of Phosphoinositides

The Use of Lipid–Binding Toxins to Study Distribution and Dynamics of Sphingolipids and Cholesterol

FlAsH Protein Labeling

AGT/SNAP–tag: A Versatile Tag for Covalent Protein Labeling

In Vivo Protein Labelling with Trimthoprim and Dihydrofolate Reductase

Phosphopantetheinyl Transferase Catalyzed Protein Labeling and Molecular Imaging

Bioorthogonal Chemical Transformations in Proteins by an Expanded Genetic Code

Using the Bacteriophage MS2 Coat Protein–RNA Binding Interaction to Visualise RNA in Living Cells
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Lawrence W. Miller
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