Biophysical Analysis of Membrane Proteins. Investigating Structure and Function

  • ID: 2179732
  • Book
  • 368 Pages
  • John Wiley and Sons Ltd
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Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is a cutting–edge resource for the major biophysical methods that are – or soon will be– the major techniques used in the field. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Each chapter is dedicated to a specific approach, describing the method involved, highlighting the experimental procedure and/or the basic principles, offering an up–to–date understanding of what is measured, what can be deduced from the measurements, as well as the limitations of each procedure.

Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopes, finishing off with an exploration of structure–function relationships in whole cells.

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Introduction

STRUCTURAL APPROACHES

Membrane Protein Structure Determination by Electron Cryo–microscopy

NMR Liquid or Solid State

MOLECULAR INTERACTION AND LARGE ASSEMBLIES

Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent

SPR Biosensors for Characterizing Membrane–associated Proteins

Atomic Force Microscopy (AFM): High Resolution Imaging of Structure and Assembly of Membrane Proteins

DYNAMICS

Molecular Dynamics Simulations

Understanding Structure and Function of Membrane Proteins Using Free Energy Calculations

Neutron Scattering to Study Membrane Structure and Dynamics

SPECTROSCOPIES

Circular Dichroism: Folding and Conformational Changes of Membrane Proteins

Membrane Protein Structure and Conformational Change Probed Using Fourier Transform Infrared Spectroscopy

Raman Spectroscopy on Light Harvesting Complex

EXPLORING STRUCTURE–FUNCTION RELATIONSHIPS IN WHOLE CELLS

Energy Transfer Technologies to Monitor the Dynamics and Signaling Properties of G–protein Coupled Receptors in Living Cells
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Eva Pebay–Peyroula
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