The Handbook of Plant Functional Genomics. Concepts and Protocols. Molecular Plant Biology

  • ID: 2179798
  • Book
  • 576 Pages
  • John Wiley and Sons Ltd
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The Present Handbook of Plant Functional Genomics (Concepts and Protocols) Spans over several aspects of current and emerging technologies in the field.

The editors – two of the leading experts in research with a proven track record – combine their expertise to provide an invaluable reference on the topic. To make it more inclusive, this second volume in the Molecular Plant Biology Handbook Series is divided into four main parts:

  • Transcriptome Analysis
  • Gene Silencing, Mutation Analysis, and Functional Genomics
  • Computational Analysis
  • Functional Genomics and Emerging Technologies.

Each chapter introduces the subject comprehensively, and provides state–of–the art experimental protocols. A handy glossary of relevant technical terms is included to facilitate reading for researchers, teachers and students.

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TRANSCRIPTOME ANALYSIS

Single–Cell Expression Profiling

AFLP–based RNA Fingerprinting

The SAGE family: SAGE, LongSAGE and SuperSAGE

DeepCAGE: High–Throughput Gene Expression Analysis for Transcription Start Site and Promoter Identification

Paired End diTagging (GIS–PET) for Transcriptome Characterization

High–Throughput Functional Screening of Genes in planta

Microarrays as Tools to Decipher Transcriptomes in Symbiotic Interactions

BeadArray–based Gene Expression Analysis

Genome–wide Analysis of mRNA expression by Fluorescent Differential Display

Quantitative Real–time RT–PCR

GENE SILENCING, MUTATION ANALYSIS, AND FUNCTIONAL GENOMICS

RNA Interference for Functional Genomics

Extending Functional Genomics: VIGS for Model and Crop Plants

Identification of Expressed Genes by Mutation: TILLING

Identification of Expressed Genes by Mutation: Transposon Tagging

Fast Neutron Mutagenesis

COMPUTATIONAL ANALYSIS

Bioinformatics Tools to Discover Co–expressed Genes in Plants

Promoter Analysis

Integration and Phylogenomics

Structural, Functional, and Comparative Annotation of Plant Genomes

Large–Scale Genomic Sequence Comparisons and Gene Identification with ClustDB

EMERGING TECHNOLOGIES

Nanotechnologies and Fluorescent Proteins for Functional Genomics

Next Generation Sequencing Technologies

454 Sequencing for Functional Genomics
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"... I recommend this book to any plant researcher who is interested in incorporating functional genomics approaches or who is looking to expand their repertoire. I especially liked that most chapters include detailed ′recipes′, which show that anyone with a standard wet laboratory can do most of the procedures... a very useful volume..." –The Quarterly Review of Biology, March 2010

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