Proteomics in Practice. A Guide to Successful Experimental Design. 2nd, Completely Revised Edition

  • ID: 2179817
  • Book
  • 502 Pages
  • John Wiley and Sons Ltd
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A thoroughly revised edition of the successful practical guide to experimental methods in proteomics, which now includes DIGE technology and liquid chromatography.

The first part, written in a textbook style, presents the various techniques and routes required in approaching proteomic analysis. Key methods explained are electrophoretic techniques including isoelectric focusing, SDS page, 2–D page and DIGE, and liquid chromatography techniques including ion exchange, affinity chromatography and reversed–phase HPLC. Mass–spectrometric techniques such as MALDI, ESR and FT ICR are addressed as well as a section on proteome functionality. The second part is a comprehensive, laboratory manual spanning the full range of methods from sample preparation to protein identification. The third part is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further information.

Updates of protocols as well as animations illustrating crucial methodological steps are available on the Web at [external URL]

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Preface

Foreword

Abbreviations

Glossary of terms

INTRODUCTION

History

Critical points

Hypothesis driven approach vs. High Throughput

Complementarities of workflows

Concept of experimental planning

Success stories, highlights of useful references

PART I: PROTEOMICS TECHNOLOGY

Electrophoresis

Liquid Chromatography

Mass spectrometry

Bioinformatics tools / data mining

Methods under development

PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS

Strategy where to start

Gel–based workflow

LC–based workflow

Latest trends

Validation with Western blotting

Statistic evaluation

PART III: TROUBLE SHOOTING

Gel–based workflow

LC based workflow

References

Index

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Reiner Westermeier
Tom Naven
Hans–Rudolf Höpker
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