Chemical Cytometry. Ultrasensitive Analysis of Single Cells

  • ID: 2179964
  • Book
  • 267 Pages
  • John Wiley and Sons Ltd
1 of 4
Tying together technological and conceptual advances oft he past decades, this seminal book introduces the novel approach of chemical cytometry – the biochemical analysis of cell constituents at the single cell level. As such, it combines recently developed methods for the handling of single cells, the separation of their components, and the detection and analysis of biomolecular species with application examples from cell biology, neurobiology and molecular medicine.

The editor has succeeded in acquiring contributions from the recognized leaders in the field, resulting in a complete and authoritative overview for both developers as well as users of these new methods.

From the Contents:

∗ Ultrasensitive detection of low–copy molecules from single cells

∗ Microfluidic technology for single–cell analysis

∗ On–chip electroporation and electrofusion for single–cell engineering

∗ Electrochemical determination of enzyme activity in single cells

∗ Single cell mass spectrometry

∗ Optical sensing arrays for single–cell analysis

∗ Metabolic cytometry and cell signaling studies

And many more.
Note: Product cover images may vary from those shown
2 of 4
Preface

ORIGIN, CURRENT STATUS, AND FUTURE PERSPECTIVES OF CHEMICAL CYTOMETRY

The Cell and Cytometry

Flow and Image Cytometry

Chemical Cytometry

Chemical Cytometry of DNA and mRNA

Metabolic Cytometry

Future Perspectives on Instrumentation for Chemical Cytometry

METABOLIC CYTOMETRY –

THE STUDY OF GLYCOSPHINGOLIPID METABOLISM IN SINGLE PRIMARY CELLS OF THE DORSAL ROOT GANGLIA

Introduction

Material and Methods

Results and Discussion

Conclusions

CELL SIGNALING STUDIES AT THE SINGLE–CELL LEVEL

Introduction

Analytes Examined and Reporters Used

Cell Preparation and Reporter Loading

Cell Lysis and Sampling Techniques

Electrophoresis Separation Conditions

Detection

Automation and Throughput

ULTRASENSITIVE DETECTION OF LOW–COPY–NUMBER MOLECULES FROM SINGLE CELLS

Introduction

Microchip Designs for Single–Cell Analysis and/or Cell Manipulation

Ultrasensitive Detection Methods for Single–Cell Analysis

Single–Cell Analysis with Single–Molecule Sensitivity on Integrated Microfluidic Chip

Conclusions

CAPILLARY ELECTROPHORESIS OF NUCLEIC ACIDS AT THE SINGLE–CELL LEVEL

Introduction

On–Line Cell Analysis

Direct Gene and Gene Expression Analysis Without Amplification

Potential Alternative Techniques for Single–Cell Gene and Gene Expression Analysis

Conclusions

MICROFLUIDIC TECHNOLOGY FOR SINGLE–CELL ANALYSIS

Introduction

Biological Significance of Single–Cell Analysis

Microfluidic Devices in Our Laboratories

Materials, Methods, and Protocols

Conclusions

ON–CHIP ELECTROPORATION AND ELECTROFUSION FOR SINGLE–CELL ENGINEERING

Introduction

Single–Cell Electroporation in Microfluidic Devices

Single–Cell Electrofusion in Microfluidic Devices

Conclusions

ELECTROPORATIVE FLOW CYTOMETRY FOR SINGLE–CELL ANALYSIS

Introduction

Flow–Through Electroporation under Constant Voltage

Electroporative Flow Cytometry for Detecting Protein Translocation

Electroporative Flow Cytometry for Measuring Single–Cell Biomechanics

Electroporative Flow Cytometry for Selectively Releasing and Analyzing Specific Intracellular Molecules

Conclusion

ULTRASENSITIVE ANALYSIS OF INDIVIDUAL CELLS VIA DROPLET MICROFLUIDICS

Introduction

Droplet Properties

Droplet Generation

Cell Encapsulation

Droplet Manipulation

Droplet Concentration Control

Temperature Control of Droplets

Detection in Droplets

Conclusions

PROBING EXOCYTOSIS AT SINGLE CELLS USING ELECTROCHEMISTRY

Introduction

Measurement Requirements

Electrode Fabrication

Measurements at Single Cells

Fusion Pore Dynamics

Conclusions

ELECTROCHEMICAL DETERMINATION OF ENZYME ACTIVITY IN SINGLE CELLS

Introduction

Electrochemical Detection Coupled with Capillary Electrophoresis

Voltammetry

Scanning Electrochemical Microscopy (SECM)

High–Throughput ECD

Perspective

SINGLE–CELL MASS SPECTROMETRY

Introduction

Mass Spectrometry

Overall Outlook for Single–Cell MS

OPTICAL SENSING ARRAYS FOR SINGLE–CELL ANALYSIS

Introduction to Fiber–Optic Single–Cell Arrays

Advantages of Fiber–Optic Single–Cell Arrays

Fiber–Optic Arrays

Singe–Cell Arrays for Bacteria

Single–Cell Arrays for Yeast

Single–Cell Arrays for Mammalian Cells

Image and Data Analysis for Single–Cell Arrays

Summary
Note: Product cover images may vary from those shown
3 of 4

Loading
LOADING...

4 of 4
Chang Lu is an associate professor of chemical engineering at Virginia Tech, Blacksburg (USA). From 2004 to 2009 he was an assistant and associate professor of biological engineering at Purdue University at West Lafayette. He obtained his academic degrees from Peking University and from the University of Illinois at Urbana–Champaign, and received post–doctoral training at Cornell University. His research has been focused on Microfluidic devices for single cell analysis and tissue engineering. Professor Lu has received a number of awards, including the NSF Career Award and the Wallace H. Coulter foundation Early Career Award in Biomedical Engineering.
Note: Product cover images may vary from those shown
5 of 4
Note: Product cover images may vary from those shown
Adroll
adroll