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Bioanalytical Chemistry

  • ID: 2181247
  • Book
  • 384 Pages
  • John Wiley and Sons Ltd
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Bioanalytical Chemistry provides a thorough introduction for students and practitioners with a broad range of backgrounds from chemistry to medicine. In so doing, it brings together many of the techniques commonly used by biochemists and molecular biologists. The text includes entire chapters on design and implementation of enzyme assays; mass spectrometry; and validation of new methods. Each chapter progresses from basic concepts to applications involving real samples, and ends with a set of problems, while an appendix contains selected answers. The authors have limited mathematical derivations to those that are essential for an understanding of each method and they include a list of suggested reading for further information. This textbook provides an ideal companion for students, researchers, and industrial scientists working in chemistry, biology, biochemistry, pharmacy, and medicine.
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1. Spectroscopic Methods for Matrix Characterization.

2. Enzymes.

3. Quantitation of Enzymes and Their Substrates.

4. Immobilized Enzymes.

5. Antibodies.

6. Quantitative Immunoassays with Labels.

7. Biosensors.

8. Directed Evolution for the Design of Macromolecular Bioassay Reagents.

9. Principles of Electrophoresis.

10. Applications of Zone Electrophoresis.

11. Isoelectric Focusing.

12. Capillary Electrophoresis.

13. Centrifugation Methods.

14. Chromatography of Biomolecules.

15. Mass Spectrometry of Biomolecules.

16. Validation of New Bioanalytical Methods.

Suggested References.


Answers to Problems.

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"…a valuable addition to the analytical literature…" (Analytical Bioanlaytical Chemistry, 15th October 2005)

"…the content of the book is presented in a very modern way, is clearly written and can be recommended…" (Chemical Analysis, September 2005)

"…an excellent, concise…resource on a wide variety of analytical tools." (Journal of Natural Products, June 2005)

"…I recommend the textbook 'Bioanalytical Chemistry' as a good start for the interested reader or for use in undergraduate courses, particularly by teaching staff." (Analytical Chemistry, October 1, 2004)

"The authors have provided a comprehensive look at various tools and techniques that are encompassed by the term 'bioanalytical chemistry.'" (Biochemistry and Molecular Education, September/ October 2004)

"…a valuable resource for practitioners in the fields of biochemistry and molecular biology…it would also make an excellent graduate-level course resource…" (CHOICE, October 2004)

"…the authors have succeeded in providing a concise but complete practical presentation of all major analytical techniques used in biochemistry. This valuable contribution fits its target niche and audience very well." (Journal of Chemical Education, September 2004)
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