Mass Spectrometry: Instrumentation, Interpretation, and Applications enables students to become fully versed in the principles and uses of mass spectrometry. Featuring contributions from international experts, the text introduces the many perspectives and approaches that different scientific fields bring to mass spectrometry, including applications for organic and inorganic chemistry, forensic science, biotechnology, and much more. This multidisciplinary approach enables students to apply their knowledge in their chosen fields of research in order to identify, quantify, and determine the structures and chemical properties of compounds.
This text is divided into three parts that guide students from basic principles to applications:
Part One, Instrumentation, begins with basic definitions and explanations followed by a discussion of the mass spectrometer and its building blocks. Next, the text describes fragmentation methods and tandem MS analyzer configurations, ending with a short summary of separation methods used in conjunction with mass spectrometry.
Part Two, Interpretation, explains basic concepts in mass spectra interpretation and then demonstrates how these concepts are used to interpret mass spectra in organic chemistry. Students also learn how to use mass spectrometry as a tool for peptide sequencing and how to optimize sensitivity and specificity in mass spectrometric proteome analysis.
Part Three, Applications, features ten researchers and research groups from different fields describing how they use mass spectrometry in their own work.
Designed for graduate–level students, this textbook assumes that most students will not become mass spectrometry specialists. Instead, it focuses on how they can use the mass spectrometer to support and advance research across a broad range of disciplines.
PART I INSTRUMENTATION.
1 DEFINITIONS AND EXPLANATIONS (Ann Westman–Brinkmalm and Gunnar Brinkmalm).
2 A MASS SPECTROMETER S BUILDING BLOCKS (Ann Westman–Brinkmalm and Gunnar Brinkmalm).
2.1. Ion Sources.
2.2. Mass Analyzers.
3 TANDEM MASS SPECTROMETRY (Ann Westman–Brinkmalm and Gunnar Brinkmalm).
3.1. Tandem MS Analyzer Combinations.
3.2. Ion Activation Methods.
4 SEPARATION METHODS (Ann Westman–Brinkmalm, Jerzy Silberring, and Gunnar Brinkmalm).
4.2. Electric–Field Driven Separations.
PART II INTERPRETATION.
5 INTRODUCTION TO MASS SPECTRA INTERPRETATION: ORGANIC CHEMISTRY (Albert T. Lebedev).
5.1. Basic Concepts.
5.2. Inlet Systems.
5.3. Physical Bases of Mass Spectrometry.
5.4. Theoretical Rules and Approaches to Interpret Mass Spectra.
5.5. Practical Approaches to Interpret Mass Spectra.
6 SEQUENCING OF PEPTIDES AND PROTEINS (Marek Noga, Tomasz Dylag, and Jerzy Silberring).
6.1. Basic Concepts.
6.2. Tandem Mass Spectrometry of Peptides and Proteins.
6.3. Peptide Fragmentation Nomenclature.
6.4. Technical Aspects and Fragmentation Rules.
6.5. Why Peptide Sequencing?
6.6. De Novo Sequencing
6.7. Peptide Derivatization Prior to Fragmentation.
7 OPTIMIZING SENSITIVITY AND SPECIFICITY IN MASS SPECTROMETRIC PROTEOME ANALYSIS (Jan Eriksson and David Fenyö).
7.2. Peptide and Protein Identification.
7.3. Success Rate and Relative Dynamic Range.
PART III APPLICATIONS.
8 DOPING CONTROL (Graham Trout).
9 OCEANOGRAPHY (R. Timothy Short, Robert H. Byrne, David Hollander, Johan Schijf, Strawn K. Toler, and Edward S. VanVleet).
10 OMICS APPLICATIONS (Simone Koñig).
10.2. Genomics and Transcriptomics.
11 SPACE SCIENCES (Robert Sheldon).
11.4. The Space MS Paradox.
11.5. A Brief History of Space MS.
11.6. GENESIS and the Future.
12 BIOTERRORISM (Vito G. DelVecchio and Cesar V. Mujer).
12.1. What is Bioterrorism?
12.2. Some Historical Accounts of Bioterrorism.
12.3. Geneva Protocol of 1925 and Biological Weapons Convention of 1972.
12.4. Categories of Biothreat Agents.
12.6. MS Identification of Biomarker Proteins.
12.7. Development of New Therapeutics and Vaccines Using Immunoproteomics.
13 IMAGING OF SMALL MOLECULES (Ma gorzata Iwona Szynkowska).
13.1. SIMS Imaging.
13.2. Biological Applications (Cells, Tissues, and Pharmaceuticals).
13.6. The Future.
14 UTILIZATION OF MASS SPECTROMETRY IN CLINICAL CHEMISTRY (Donald H. Chace).
14.2. Where are Mass Spectrometers Utilized in Clinical Applications?
14.3. Most Common Analytes Detected by Mass Spectrometers.
14.4. Multianalyte Detection of Clinical Biomarkers, The Real Success Story.
14.5. Quantitative Profiling.
14.6. A Clinical Example of the Use of Mass Spectrometry.
14.7. Demonstrations of Concepts of Quantification in Clinical Chemistry.
15 POLYMERS (Maurizio S. Montaudo).
15.2. Instrumentation, Sample Preparation, and Matrices.
15.3. Analysis of Ultrapure Polymer Samples.
15.4. Analysis of Polymer Samples in which all Chains Possess the Same Backbone.
15.5. Analysis of Polymer Mixtures with Different Backbones.
15.6. Determination of Average Molar Masses.
16 FORENSIC SCIENCES (Maria Kala).
16.2. Materials Examined and Goals of Analysis.
16.3. Sample Preparation.
16.4. Systematic Toxicological Analysis.
16.5. Quantitative Analysis.
16.6. Identification of Arsons.
17 NEW APPROACHES TO NEUROCHEMISTRY (Jonas Bergquist, Jerzy Silberring, and Rolf Ekman).
17.2. Why is there so Little Research in this Area?
17.3. Proteomics and Neurochemistry.
PART IV APPENDIX.