An extensive compilation of reviews, opinions, and applications by today′s top researchers and professionals in the field, Principles of Mass Spectrometry Applied to Biomolecules presents the latest information on the basic principles of mass spectrometry as they relate to the life sciences, with special focus on the inherent challenges posed by varying degrees of biomolecular size and flexibility.
Featuring the professional contributions of more than twenty–five experts from academia and industry, the text helps readers to understand how to use recent advances while recognizing and discussing challenges associated with analysis of biomolecules using mass spectrometry.
Topics have been arranged into three distinct sections Structures and Dynamics of Gas–Phase Biomolecules; Activation, Dissociation, and Reactivity; and Thermochemistry and Energetics and include:
- Energetics and mechanisms of peptide fragmentation
- Electron capture dissociation
- Ion–ion and ion–molecule reactions
- Reaction dynamics
- Collisional activation
- Protein structure and interactions
This important work provides today′s scientists and researchers with an in–depth understanding of the fundamental principles underlying the existing analytical approaches in this exciting and growing field, complete with its successes and limitations, so that they can develop new and innovative ways of using these techniques in their own work.
STRUCTURES AND DYNAMICS OF GAS–PHASE BIOMOLECULES.
1. Spectroscopy of neutral Peptides in the Gas Phase–Structure, Reactivity, Microsolvation, Molecular Recognition (M. Gerhards).
2. Probing the Electronic Structure of Fe–S Clusters Using Anoin Photoelectron Spectroscopy: Ubiquitous Electron Transfer Centers in Metalloproteins (X. Yang, et al.).
3. Ion/Molecule Reactions and H/D Exchange for Structural Characterization of Biomolecules (M. Green & C. Lebrilla).
4. Understanding Protein Interactions and their Representation in the Gas Phase of the Mass Spectrometer (F. Sobott & C. Robinson).
5. Protein Structure and Folding in the Gas Phase: Ubiquitin and Cytochrome c (K. Breuker).
6. Dyunamical Simulations of Photoionization of Small Biological Molecules (D. Shemesh & R. Gerber).
7. IVR and Ergodicity of Dissociation of Bio–Molecules (C. Lifshitz).
PART II: ACTIVATION, DISSOCIATION AND REACTIVITY.
8. Mechanisms of Peptide Fragmentation (V. Wysocki, et al.).
9. Peptide Radical Cations (A. Hopkinson & K. Siu).
10. Photodissociation of Biomolecule Ions: Progress, Possibilities, and the Perspectives Coming From Small–Ion Models (R. Dunbar).
11. Chemical Dynamics Simulations of EnergyTransfer and Unimolecular Decomposition in Collision–Induced Dissociation (CID) and Surface–Induced Dissociation (SID) (A. Rahaman, et al.).
12. Ion Soft Landing: Instrumentation, Phenomena and Applications (B. Gologan, et al.).
13. Electron Capture Dissociation and Other Ion–electron Fragmentation Reactions (R. Zubarev).
14. Biomolecule Ion/Ion Reactions (S. McLuckey).
PART III: THERMOCHEMISTRY AND ENERGETICS.
15. Thermochemistry Studies of Biomolecules (C. Wesdemiotis).
16. Energy and Entropy Effects in the Gas Phase Dissociation of Peptides and Proteins (J. Laskin).