HPLC Made to Measure. A Practical Handbook for Optimization

  • ID: 2183084
  • Book
  • 786 Pages
  • John Wiley and Sons Ltd
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Alongside developing new ones, the optimization of existing methods is a key task in the HPLC laboratory. And a task that nowadays has to be solved increasingly fast and cost–efficiently.

This handbook provides well–founded assistance in better meeting this challenge. Internationally renowned authors cover both the general basics and strategies in optimization, as well as the specific aspects involved in such different methods as RP–HPLC, NP–HPLC, micro– and nano–HPLC, and hyphenated techniques, such as LC/MS. Nor are such topics as column selection and chiral separations left uncovered. Some of the contributions present applications using common optimization software, such as DryLab or ChromSword. All of the chapters concentrate on the essentials and are written in a practically oriented style, while their self–contained structure allows for targeted references.

Throughout the text, the authors offer concrete, practical tips as well as pertinent background information, together with insights into the optimization methods of seven major international companies from various sectors.

The whole is rounded off with real–life reports from experienced users coming from the different areas of application, in particular the life sciences, such as proteomics or drug development.
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ESSENTIAL ASPECTS OF OPTIMIZATION

Principles of HPLC Optimization Illustrated by RP–Chromatography

Fast Gradient Separations

pH and Selectivity in Reversed–Phase Chromatography

Selecting the Correct pH–Value for HPLC

Optimization of Data Evaluation in Chromatography

Calibration Characteristics and Uncertainty indicating Starting–Points for Method Optimization

OPTIMIZATION IN DIFFERENT LC–MODI

Comparison and Choice of Columns for RP–HPLC

Column Selectivity in Reversed–Phase Chromatography

The Use of Principal Component Analysis for the Characterisation of Reversed–Phase Liquid Chromatographic Stationary Phases

Chemometrics –

A Powerful Tool for Handling a Large Number of Data

Linear Free Energy Relations –

Tools for Column Characterization and Method Optimization in HPLC?

Column Selectivity in Reversed–Phase Liquid Chromatography

Understanding Selectivity by Use of Suspended–State High Resolution Magic Angle Spinning NMR Spectroscopy

Optimization in Normal–Phase HPLC

Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection

Gel Filtration –

Size Exclusion Chromatography of biopolymers

Optimization in Affinity Chromatography

Optimization of Chiral Separations in HPLC

µLC/nano LC –

Optimization and Troubleshooting

Microchip–Based Liquid Chromatography –

Techniques and Possibilities

Ultra–Performance Liquid Chromatography

HYPHENATED TECHNIQUES

Immunochromatographic Techniques

Enhanced Characterization and Comprehensive Analyses by 2–dimensional Chromatography

LC/MS –

Hints and Recommendations for Optimization and Troubleshooting

LC–NMR–Coupling

COMPUTER–AIDED OPTIMIZATION

Computer–Facilitated HPLC Method Development Using DryLab Software

ChromSword Software for Automated and Computer–Assisted Development of HPLC Methods

Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC

USER REPORTS

NanoLC–MS/MS in Proteomics

Ways for the Verification of the Robustness in RP–HPLC

Separation of Complex Sample Mixtures

Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC–UV/MS/CLND
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"A valuable text by any estimation, I would recommend it highly for hands–on users or managers directing a group of chromatographers toward more efficient and effective HPLC separations." (Journal of the American Chemical Society, February 21, 2007)
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