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Protein Chromatography. Process Development and Scale-Up

  • ID: 2183150
  • Book
  • 364 Pages
  • John Wiley and Sons Ltd
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A practice–oriented all–in–one guide that provides the necessary knowledge for effective process development in chromatographic bioseparation, both for preparative–scale and for large–scale industrial applications.

With its focus on process development and bioseparation tasks, this is tailor–made reading for the professional bioengineer in both the biotech and pharmaceutical industries. Following a tried and–tested concept, this guide has been developed over several years in training courses for biotech and chemical engineers in Europe and the U.S. Coverage begins with an overview of biomolecular properties and their relationship to downstream processing, followed by a detailed treatment of bioprocess chromatography, its dynamics, the mass transfer and dispersion effects that control its performance, the critical design and scale–up relationships, as well as the relevant stationary phases and equipment.
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Preface

DOWNSTREAM PROCESSING OF BIOTECHNOLOGY PRODUCTS

Introduction

Bioproducts and their Contaminants

Bioprocesses

Role of Chromatography in Downstream Processing

References

INTRODUCTION TO PROTEIN CHROMATOGRAPHY

Introduction

Basic Principles and Definitions

Modes of Operation

Performance Factors

Separation Performance Metrics

CHROMATOGRAPHY MEDIA

Introduction

Interaction Types and Chemistry

Buffers and Mobile Phases

Physical Structure and Properties

LABORATORY AND PROCESS COLUMNS AND EQUIPMENT

Introduction

Laboratory–scale Systems

Process Columns and Equipment

ADSORPTION EQUILIBRIA

Introduction

Single Component Systems

Multi–component Systems

References

ADSORPTION KINETICS

Introduction

Rate Mechanisms

Batch Adsorption Kinetics

DYNAMICS OF CHROMATOGRAPHY COLUMNS

Introduction

Conservation Equations

Local Equilibrium Dynamics

Multi–component Systems

Displacement Development

EFFECTS OF DISPERSION AND ADSORPTION KINETICS ON COLUMN PERFORMANCE

Introduction

Empirical Characterization of Column Efficiency

Modeling and Prediction of Column Efficiency

GRADIENT ELUTION CHROMATOGRAPHY

Introduction

General Theory for Gradient Elution with Linear Isotherms

LGE Relationships for Ion Exchange Chromatography

LGE Relationships for RPC and HIC

Separations with pH Gradients

Modeling Gradient Elution with Non–linear Isotherms

References

DESIGN OF CHROMATOGRAPHIC PROCESSES

Introduction

Chromatographic Process Steps and Constraints

Design for Capture

Design for Chromatographic Resolution

SMB Design

References

INDEX

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Giorgio Carta
Alois Jungbauer
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