FRET - Förster Resonance Energy Transfer. From Theory to Applications

  • ID: 2183347
  • Book
  • 816 Pages
  • John Wiley and Sons Ltd
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Meeting the need for an up–to–date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors.

The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET.

Written for both the FRET novice and for the seasoned user, this is a must–have resource

for office and laboratory shelves.

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Preface

PART ONE: BACKGROUND, THEORY, AND CONCEPTS

How I Remember Theodor Förster

Remembering Robert Clegg and Elizabeth Jares–Erijman and Their Contributions to FRET

Förster Theory

Optimizing the Orientation Factor Kappa–Squared for More Accurate FRET Measurements

How to Apply FRET: From Experimental Design to Data Analysis

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

PART TWO: COMMON FRET TECHNIQUES/APPLICATIONS

In Vitro FRET Sensing, Diagnostics, and Personalized Medicine

Single–Molecule Applications

Implementation of FRET Technologies for Studying the Folding and Conformational Changes in Biological Structures

FRET–Based Cellular Sensing with Genetically Encoded Fluorescent Indicators

PART THREE: FRET WITH RECENTLY DEVELOPED MATERIALS

FRET with Fluorescent Proteins

Semiconductor Quantum Dots and FRET

Multistep FRET and Nanotechnology

PART FOUR: SUPPORTING INFORMATION AND CONCLUSIONS

Data

Outlook on FRET: The Future of Resonance Energy Transfer

Index
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Igor L. Medintz
Niko Hildebrandt
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