The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET.
Written for both the FRET novice and for the seasoned user, this is a must–have resource
for office and laboratory shelves.
PART ONE: BACKGROUND, THEORY, AND CONCEPTS
How I Remember Theodor Förster
Remembering Robert Clegg and Elizabeth Jares–Erijman and Their Contributions to FRET
Optimizing the Orientation Factor Kappa–Squared for More Accurate FRET Measurements
How to Apply FRET: From Experimental Design to Data Analysis
Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations
PART TWO: COMMON FRET TECHNIQUES/APPLICATIONS
In Vitro FRET Sensing, Diagnostics, and Personalized Medicine
Implementation of FRET Technologies for Studying the Folding and Conformational Changes in Biological Structures
FRET–Based Cellular Sensing with Genetically Encoded Fluorescent Indicators
PART THREE: FRET WITH RECENTLY DEVELOPED MATERIALS
FRET with Fluorescent Proteins
Semiconductor Quantum Dots and FRET
Multistep FRET and Nanotechnology
PART FOUR: SUPPORTING INFORMATION AND CONCLUSIONS
Outlook on FRET: The Future of Resonance Energy Transfer
Professor Niko Hildebrandt obtained a Diploma in Medical Physics in 2001 at the University of Applied Sciences Berlin and a Ph.D. degree in Physical Chemistry in 2007 at the University of Potsdam, where he also carried out postdoctoral research until 2008. From 2008 to 2010 he was head of the group NanoPolyPhotonics at the Fraunhofer Institute for Applied Polymer Research in Potsdam. Since 2010 he has been Full Professor at Université Paris–Sud, where he is leading the group of NanoBioPhotonics (www.nbp.ief.u–psud.fr) at the Institut d′Electronique Fondamentale with a research focus on time–resolved FRET spectroscopy and imaging for multiplexed nanobiosensing.