Understanding DNA and Gene Cloning. A Guide for the Curious. 4th Edition

  • ID: 2240463
  • Book
  • 370 Pages
  • John Wiley and Sons Ltd
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A LAYMAN S TOUR OF GENETICS FROM MOLECULAR BASICS TO GENE CLONING AND ITS APPLICATIONS

Will genetic matching free your children of hereditary disease? Will insurance companies deny your life insurance because of predetermined patterns in your DNA?  Will the perfect potato ever make the ultimate–tasting French fry?

This highly acclaimed book fully explains the genetic issues we face today and will face tomorrow, and guides you through the science and technology underlying the ongoing genetic revolution. Each step of the way, Karl Drlica explains complex topics using easy–to–read examples and understandable prose. This basic information will help you:

  • Understand the basics of molecular biology and DNA technology so you can make informed judgments about these topics.
  • Realize the benefits and shortcomings of emerging advances in genetics to change your life for the better.
  • Protect yourself from professional and personal discrimination that may arise from the release of genetic information.
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Chapter 1. Preview: Life as Interacting Molecules.

PART I. BASIC MOLECULAR GENETICS.

Chapter 2. Structure of DNA: Two Long, Interwound Chains.

Chapter 3. Reproducing DNA: Information Transfer from One Generation to the Next.

Chapter 4. Gene Expression: Cellular Use of Genetic Information.

PART II. MANIPULATION DNA.

Chapter 5. Microbial Growth: One–celled Organisms as Tools for Gene Cloning.

Chapter 6. Plasmids and Phages: Submicroscopic Parasites Used to Deliver Genes to Cells.

Chapter 7. Cutting and Joining DNA: Restriction Endonucleases and Ligases Used to Restructure DNA Molecules.

Chapter 8. Using Complementary Base Pairing: Hybridization, Probes, and Amplification using PCR.

Chapter 9. Cloning Genes: Isolation of a Hemoglobin Gene.

Chapter 10. Using Cloned Genes: Gene Structure, Expression Vectors, and Gene Function.

PART III. MODERN MOLECULAR GENETICS.

Chapter 11. Beyond the Central Dogma: A Sampling of Insights Derived from Gene Cloning.

Chapter 12. Retroviruses: AIDS and Gene Delivery.

Chapter 13. Cancer Genes: The Loss of Growth Control.

PART IV: HUMAN GENETICS.

Chapter 14. Heredity: Pattern of Inheritance and Genetic Instability.

Chapter 15. Application of Human Genetics: Genetic Testing, Gene Therapy, and DNA Fingerprinting.

PART V. WHOLE GENOMES.

Chapter 16. Genomics: Using Whole Genomes to Study Life.

Additional Reading.

Glossary.

Abbreviations.

Index.

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Karl Drlica
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