DNA Sequencing Strategies. Automated and Advanced Approaches

  • ID: 2245377
  • Book
  • 202 Pages
  • John Wiley and Sons Ltd
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For the past seven years, a few scientists each year have had the privilege of attending the European Molecular Biology Organization (EMBO) practical course on DNA sequencing. There they learned the latest strategies, techniques, and protocols for taking full advantage of automated sequencing technologies and advanced biochemical approaches. Developed from the book of protocols used in the EMBO course, DNA Sequencing Strategies enables researchers the world over to reduce random sequencing failures and increase the number of bases in the readable sequence.

This user–friendly laboratory bench reference leads you step by step through the sequencing process, pointing out critical choices, refining techniques, and connecting particular strategies with specific circumstances and research requirements. Topics covered include:

∗ Preparation of DNA for sequencing––discussion of various sequencing strategies to consider before preparation begins

∗ Template preparation––different approaches required for the preparation of various quantities of DNA

∗ Protocols for sequencing single–stranded and double–stranded DNA as well as cycle sequencing

∗ Selection, synthesis, and purification of primers

∗ Preparation of sequencing gels and the actual collection of results

∗ Labeling of DNA fragments for sequencing

∗ Computer analysis of sequencing data

Supplemented with more than 100 data charts, tables, and photographs, this is the ultimate guide for industry professionals, researchers, and students in biotechnology, molecular biology, and biochemistry.
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Sequencing Strategies and Cloning Protocols.

Preparation of Templates.

DNA Sequencing Protocols.

Selection, Synthesis, and Purification of Primers.

Preparation of DNA Sequencing Gels and Electrophoresis.

Computer Analysis.

Index.
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Wilhelm Ansorge
Hartmut Voss
Jürgen Zimmermann
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