This user–friendly laboratory bench reference leads you step by step through the sequencing process, pointing out critical choices, refining techniques, and connecting particular strategies with specific circumstances and research requirements. Topics covered include:
∗ Preparation of DNA for sequencing––discussion of various sequencing strategies to consider before preparation begins
∗ Template preparation––different approaches required for the preparation of various quantities of DNA
∗ Protocols for sequencing single–stranded and double–stranded DNA as well as cycle sequencing
∗ Selection, synthesis, and purification of primers
∗ Preparation of sequencing gels and the actual collection of results
∗ Labeling of DNA fragments for sequencing
∗ Computer analysis of sequencing data
Supplemented with more than 100 data charts, tables, and photographs, this is the ultimate guide for industry professionals, researchers, and students in biotechnology, molecular biology, and biochemistry.
Preparation of Templates.
DNA Sequencing Protocols.
Selection, Synthesis, and Purification of Primers.
Preparation of DNA Sequencing Gels and Electrophoresis.