The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice – which is becoming ever more important even in classical organic synthesis!
Evolutionary Biotechnology –
From Ideas and Concepts to Experiments and Computer Simulations
Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases
Construction of Environmental Libraries for Functional Screening of Enzyme Activity
Investigation of Phage Display for the Directed Evolution of Enzymes
Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process
Yeast n–Hybrid Systems for Molecular Evolution
Advanced Screening Strategies for Biocatalyst Discovery
Engineering Protein Evolution
Exploring the Diversity of Heme Enzymes through Directed Evolution
Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry
Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA
Evolutionary Generation versus Rational Design of Restriction Endonucleases with Novel Specificity
Evolutionary Generation of Enzymes with Novel Substrate Specificities
Kai Johnsson is assistant professor for Bioorganic Chemistry at the Swiss Federal Institute for Technology (EPFL) where he heads the laboratory for protein engineering. Prior to joining EPFL, he was a junior group leader at Ruhr–University Bochum in Germany, after spending three and a half years in the laboratory of Prof. Peter G. Schultz (University of California, Berkeley) as a postdoctoral research fellow.
Kai Johnsson studied chemistry and did his PhD with Prof. Steven Benner at ETH Zurich. Since the start of his PhD thesis, Kai Johnsson′s research interests focus on biological chemistry and in particular enzyme mechanisms and protein chemistry. Prof. Johnsson is the inventor of the Covalys technology.