Directed Molecular Evolution of Proteins. Or How to Improve Enzymes for Biocatalysis

  • ID: 2293073
  • Book
  • 368 Pages
  • John Wiley and Sons Ltd
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Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio– and enantioselectivity.

The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice – which is becoming ever more important even in classical organic synthesis!
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Introduction

Evolutionary Biotechnology –

From Ideas and Concepts to Experiments and Computer Simulations

Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases

Construction of Environmental Libraries for Functional Screening of Enzyme Activity

Investigation of Phage Display for the Directed Evolution of Enzymes

Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process

Yeast n–Hybrid Systems for Molecular Evolution

Advanced Screening Strategies for Biocatalyst Discovery

Engineering Protein Evolution

Exploring the Diversity of Heme Enzymes through Directed Evolution

Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry

Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA

Evolutionary Generation versus Rational Design of Restriction Endonucleases with Novel Specificity

Evolutionary Generation of Enzymes with Novel Substrate Specificities
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Susanne Brakmann
Kai Johnsson
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