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Directed Molecular Evolution of Proteins. Or How to Improve Enzymes for Biocatalysis. Edition No. 1

  • ID: 2293073
  • Book
  • March 2002
  • 368 Pages
  • John Wiley and Sons Ltd
Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity.
The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!
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Evolutionary Biotechnology -
From Ideas and Concepts to Experiments and Computer Simulations
Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases
Construction of Environmental Libraries for Functional Screening of Enzyme Activity
Investigation of Phage Display for the Directed Evolution of Enzymes
Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process
Yeast n-Hybrid Systems for Molecular Evolution
Advanced Screening Strategies for Biocatalyst Discovery
Engineering Protein Evolution
Exploring the Diversity of Heme Enzymes through Directed Evolution
Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry
Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA
Evolutionary Generation versus Rational Design of Restriction Endonucleases with Novel Specificity
Evolutionary Generation of Enzymes with Novel Substrate Specificities
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Susanne Brakmann University of Leipzig, Germany.

Kai Johnsson Department of Chemistry, Inst. of Organic Chemistry, EPFL - BCH, Switzerland.
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