This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation.
Luminous Marine Organisms
Beetle Luciferases: Colorful Lights on Biological Processes and Diseases
Split Luciferase Systems for Detecting Protein–Protein Interactions in Mammalian Cells
Photoproteins in Nucleic Acid Analysis
Bioluminescence Resonance Energy Transfer in Bioanalysis
Photoproteins as in Vivo Indicators of Biological Function
Photoproteins as Reporters in Whole–cell Sensing
Luminescent Proteins in Binding Assays
Applications in Microfluidics and Miniaturized Analytical Systems
Advances in Instrumentation for Detecting Low–level Bioluminescence and Fluorescence
Photoproteins and Instrumentation: Their Availability and Applications in Bioanalysis
Sapna K. Deo is Assistant Professor at the Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis. She gained her PhD in bioanalytical chemistry from the University of Kentucky in 2000,where she also held the posts of postdoctoral fellow and assistant research professor. Dr. Deo has co–organized a symposium for the American Chemical Society, of which she is a member. She is a reviewer for several journals in the field.