Proteomics Sample Preparation

  • ID: 2326672
  • Book
  • 485 Pages
  • John Wiley and Sons Ltd
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This long–awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles –– sample, method and aim of the study –– this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step–by–step protocols adaptable to any specific proteome analysis task.
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PERSPECTIVES IN PROTEOMICS SAMPLE PREPARATION

Introduction

General Aspects of Sample Preparation for Comprehensive Proteome Analysis

Proteomics: A Philosophical Perspective

MASS SPECTROMETRY

A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application

Sample Preparation for the Application of MALDI Mass Spectrometry

Sample Preparation for Label–Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry

Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr

Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging

ELECTROPHORESIS

Sample Preparation for Two–Dimensional Gel Electrophoresis

Sample Preparation for Native Electrophoresis

Sample Preparation for LC–MS/MS using Free Flow Electrophoresis

Sample Preparation for Proteomics

OPTICAL METHODS

High–Throughout Proteomics: Spinning Disc Interferometry (SDI)

Optical Proteomics on Cell Arrays

Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis

Sample Preparation for Flow Cytometry

CHROMATOGRAPHY

Sample Preparation for HPLC–Based Proteome Analysis

Sample Preparation for Two–Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis

STRUCTURAL PROTEOMICS

Exploring Protein–Ligand Interactions by Solution NMR

Sample Preparation for Crystallography

INTERACTION ANALYSIS

Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP)

Methods

Exploring Membrane Proteomes

POST–TRANSLATIONAL MODIFICATIONS

Sample Preparation for Phosphoproteome Analysis

Sample Preparation for Analysis of Post–Translational Modifications: Glycosylation

SPECIES–DEPENDENT PROTEOMICS

Sample Preparation and Data Processing in Plant Proteomics

Sample Preparation for MudPIT with Bacterial Protein Samples

Sample Preparation for the Cell–Wall Proteome Analysis of Yeast and Fungi

THE HUMAN PROTEOSOME

Clinical Proteomics: Sample Preparation and Standardization

Stem Cell Proteomics

BIOINFORMATICS

Bioinformatics Support for Mass Spectrometric Quality Control

Use of Physio–Chemical Properties in Peptide and Protein Identification

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Jörg von Hagen
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