Essential Guide to Reading Biomedical Papers. Recognising and Interpreting Best Practice

  • ID: 2329324
  • Book
  • 350 Pages
  • John Wiley and Sons Ltd
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Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practiceis an indispensable companion to the biomedical literature. This concise, easy–to–follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature.  Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine.

  • Allows the reader to develop the necessary skills to properly evaluate research articles
  • Coverage of over 30 commonly–used techniques in the biomedical sciences
  • Global approach and application, with contributions from leading experts in diverse fields
Appraisal of research papers in undergraduate courses tends to address the results, but rarely includes a critical evaluation of the researchers methodology. In reality, few bioscientists can claim a working knowledge of more than a handful of the techniques covered in this collection. These shortcomings can be greatly overcome thanks to this book. The contributors are active research scientists of high quality. Each addresses the methods in a critical sense and provide an expert′s view of the advantages and pitfalls. There is no equivalent book currently available. Dr David J. Miller, on behalf of The Physiological Society.
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List of contributors ix

Foreword xi

Preface xiii

Acknowledgements xvii

Introduction xix

Section A – Basic principles 1

1 Philosophy of science 3James Ladyman

2 Ingredients of experimental design 9Nick Colegrave

3 Statistics: a journey that needs a guide 17Gordon Drummond

Section B – Cell and molecular 27

4 Organ bath pharmacology 29Emma Robinson

5 Small vessel myography 39Tomoko Kamishima and John M Quayle

6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49Scott H Randell

7 Electron microscopy (TEM and SEM) 59Paul Verkade

8 Fluorescence microscopy 67Mark Jepson

9 Intracellular ′sharp′ microelectrode recording 77Helena C Parkington and Harold A Coleman

10 Single electrode voltage–clamp (SEVC) 85Harold A Coleman and Helena C Parkington

11 Patch clamp recording 95Neil Bannister and Phil Langton

12 Production of antibodies 105Elek Molnar

13 Immunocytochemistry and immunohistochemistry 117Elek Molnar

14 Immunoprecipitation (IP) 129David Bates

15 Immunoblotting (western) 137Samantha F. Moore, Joshua S. Savage and Ingeborg Hers

16 Applications of green fluorescent protein (GFP) 147Mark Jepson

17 Fluorescent measurement of ion activity in cells 153Helen Kennedy

18 Detection of exocytosis real time 163Anja Teschemacher

19 Viral vector transgenesis 173Anja Teschemacher

20 Polymerase chain reaction (PCR) and reverse transcription (RT)–PCR 179Lucy F. Donaldson

21 In situ hybridisation (ISH) 187Lucy F. Donaldson

22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193
Allison Fulford

23 Transcriptome analysis: microarrays 203Charles Hindmarch

24 Experimental proteomics 215Thierry Le Bihan

Section C – In vivo / integrative 229

25 Behavioural methodology 231Emma Robinson

26 Genetically modified mouse models 241Nina Balthasar

27 Wireless recording of cardiovascular signals 247Julian FR Paton and Fiona D McBryde

28 Electrical stimulation methods 253Jon Wakerley

29 Extracellular recording 261Jon Wakerley

30 Antidromic identification 271Jon Wakerley

31 Event–triggered averaging, including spike–triggered averaging 279Richard Apps

32 Axonal transport tracing of CNS pathways 285John Crabtree

33 Cardiovascular methods: general considerations for human studies 291Erica A Wehrwein and Michael J Joyner

34 Measuring cardiac output in humans 299Erica A Wehrwein and Michael J Joyner

35 Measuring peripheral blood flow in humans 311Erica A Wehrwein and Michael J Joyner

Index 319

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Philip D. Langton
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