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Microtubules, in vitro, Vol 115. Edition No. 2. Methods in Cell Biology

  • ID: 2534652
  • Book
  • October 2013
  • 440 Pages
  • Elsevier Science and Technology

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

  • Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research
  • Combines classical approaches and modern technologies
  • Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

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  1. Isolation of Antarctic fish tubulin and MAPs
  2. Making, isolating and characterizing tubulin alpha antibodies
  3. Techniques for labeling tubulin and drugs
  4. Single-particle 3D reconstruction methods for decorated and undecorated microtubules
  5. A new microtubule gliding assay and analysis of microtubule persistence length
  6. Characterization of gamma tubulin
  7. Biochemical and Structural Studies of the doublecortin family of MAPs
  8. Detection and quantification of microtubule detachment from centrosomes and spindle poles
  9. Regulation of tubulin expression by micro RNAs: Implications for Drug Resistance
  10. A portable magnetic tweezers device to visualize three-dimensional deformation of reconstituted microtubule networks
  11. Mitochondrial/microtubule interactions
  12. Going Solo: Measuring the motions of microtubules with an in vitro assay for TIRF microscopy
  13. Analysis of Microtubules and Motors in Isolated Axoplasm from the Squid Giant Axon
  14. Analysis of drug binding to microtubules and steady state microtubule growth/treadmilling by use of a sedimentation assay in vitro
  15. Specific recombinant antibody technology for the study of microtubule dynamics and regulatory proteins
  16. Tubulin-specific Chaperones: Components of a Molecular Machine that Assembles the aB ?Heterodimer
  17. G-protein tubulin interactions
  18. Katanin, a microtubule severing enzyme: expression, purification, and biophysical characterization
  19. Measuring tubulin polymerization in vitro
    comparison of methods and possible artifacts
  20. Imaging microtubules in living plant cells
  21. How to use which antibody known in the field, what to expect from this antibody and how semi-quantitative data can be reliably obtained with antibody staining
  22. Preparation of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences
  23. Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry
  24. TBC
  25. Characterization of the mechanism of action of the covalent-binding microtubule-stabilizing agent zampanolide
  26. Studying kinetochore-fibre ultrastructure using correlative light-electron microscopy
  27. Fluorescence-based Assays for Microtubule Structure
  28. Yeast Tubulin-structure-function analysis
  29. Microtubule Binding SIm to simulate binding of proteins to polymers under different binding models
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Correia, John J.
Wilson, Leslie
University of California, Santa Barbara, USA
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