This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling.
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers endosome signaling
- Contains chapters on such topics as measurement of biological effects of endosomal proteolysis of internalized insulin and multi-vesicular endosome biogenesis.
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Section I. COMPARTMENTS 1. Multivesicular endosome biogenesis 2. An anomalous endocytic compartment in fibroblasts from Sandhoff disease patients 3. Visualizing of signaling proteins on endosomes using Knock Down and Rescue approach 4. Direction of viral infections to unproductive compartments in endosomal signaling 5. Zinc in and out of organelles of the secretory and endosomal-lysosomal system 6. Interactions between endosomal maturation and autophagy: analysis of ESCRT machinery during C. elegans development 7. Assessment of cation trapping by cellular acidic compartments 8. Signaling from inside as initiated by the secretory compartments 9. Recycling endosomal receptors
Section II. TRANSPORT AND TRANSFER 10. Polarity proteins and endosomal protein trafficking and signaling 11. Membrane trafficking pathway from recyling endosomes to lysosomes 12. Measurement of intercellular transfer to signaling endosomes 13. Measurement of bidirectional transport between the trans-Golgi network and the endosomal system 14. Mutant mice with defects in P1(3.5)P2 biosynthesis and endosome/lysosome trafficking 15. Endosomal trafficking of the somatostatin receptor 3
Section III. PROTEINS 16. Notch 17. Endocytic regulation of notch signaling during development 18. Tollip participates in intracellular transport via endosomes 19. PX-FERM proteins