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Laboratory Methods in Enzymology: RNA, Vol 530

  • ID: 2685470
  • Book
  • 472 Pages
  • Elsevier Science and Technology
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on RNA

Please Note: This is an On Demand product, delivery may take up to 11 working days after payment has been received.

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Computational Prediction of RNA Secondary Structure

Reverse-transcription PCR (RT-PCR)

Northern blotting

Explanatory Chapter: Nuclease Protection Assays

In Vitro Transcription from Plasmid or PCR-amplified DNA

Measurement of In Vivo RNA Synthesis Rates

Method for measuring mRNA decay rate in Saccharomyces cerevisiae

Analysis of polysomes from bacteria

Polysome Profile Analysis
Yeast

Polysome analysis of mammalian cells

Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'; ends, and poly(A) tails of RNA

RNA Radiolabeling

Fluorescently Labeling Synthetic RNAs

Analysis of RNA by analytical polyacrylamide gel electrophoresis

RNA purification by preparative polyacrylamide gel electrophoresis

Explanatory Chapter: Nucleic Acid Concentration Determination

RNA purification
precipitation methods

Reverse Transcriptase Dideoxy Sequencing of RNA

RNA Structure Experimental Analysis
Chemical Modification

Structural Analysis of RNA Backbone Using In-Line Probing

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Lorsch, Jon
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