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Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

  • ID: 2857090
  • Book
  • April 2014
  • Elsevier Science and Technology

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.

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General Notes

DNA and RNA Extraction Protocols

Agarose Gell Electrophoresis 

Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)

Antibodies and Titrations

Protein Extraction

Immunoblotting (Western Blot)


Perfusion and Immersion Fixation 


In situ Hybridization

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John T. Corthell University of Michigan, Ann Arbor, MI, USA.

Dr. Corthell received a PhD in Neuroscience from Florida State University, where he studied circadian rhythms using whole-cell patch-clamp electrophysiology, as well as numerous molecular biology techniques, in the olfactory system. He has more than 10 years of experience in laboratory work and techniques in the biological and chemical sciences, and more than 5 years teaching. The author has cultivated these techniques over the years through reading, asking questions, and trial and error. Each of these techniques has been tested, performed, and in some cases optimized by the author himself. He enjoys reading, writing, learning, children, and big dogs. When not working, he longs to go SCUBA diving.
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