Reactive Species Detection in Biology: From Fluorescence to Electron Paramagnetic Resonance Spectroscopy discusses the reactive oxygen species that have been implicated in the pathogenesis of various diseases, presenting theories, chemistries, methodologies, and various applications for the detection of reactive species in biological systems, both in-vitro and in-vivo.
Techniques covered include fluorescence, high performance chromatography, mass spectrometry, immunochemistry, and electron paramagnetic resonance spectroscopy. Probe design and development are also reviewed in order to advance new approaches in radical detection through synthesis, computations, or experimental applications.
- Reviews all current advances in radical detection
- Emphasizes chemical structures and reaction schemes fundamental to radical detection and identification
- Describes the uses, advantages, and disadvantages of various probe designs
- Examines new approaches to radical probe development
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Chapter 1. Introduction
Chapter 2. Chemistry of Reactive Species
Chapter 3. Reactive Species in Biological Systems
Chapter 4. Fluorescence Technique
Chapter 5. EPR Spin Trapping
Chapter 6. UV-Vis Absorption and Chemiluminescence Techniques
Chapter 7. Electrochemical, Mass Spectroscopic, Immunochemical, and Nuclear Magnetic Resonance Techniques
Prof. Villamena received his Ph.D. in chemistry from Georgetown University and joined Ohio State in 2001. He has held a number of positions there, including several years as a research scientist/principal investigator in the Center for EPR Spectroscopy and Imaging (electron paramagnetic resonance). His current research interest is in the advancement of free radical detection and identification by EPR spectroscopy focusing mainly on the development of new spin traps and probes for chemical, biological, and biomedical imaging applications. Prof. Villamena publishes and lectures widely on this subject and has chaired the Free Radicals Session at the Rocky Mountain Conference on Analytical Chemistry for the past three years. He is an ad hoc grant reviewer for NIH and international funding agencies for the development of radical probes, and regularly reviews manuscripts on radical-related topics.