Forced-Flow Layer Chromatography takes a close look at the specifics of forced-flow layer chromatography techniques, from their evolution to the nuances of using these techniques in a variety of applications where traditional thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are not as effective.
This book presents a number of variations of TLC techniques, with special emphasis on the overpressured-layer chromatography (OPLC) technique and newer developments such as the BioArena System for biomedical analysis. The versatility of these forced-flow techniques opens up new avenues for the analysis of a large number of samples for high-throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis.
- Details a variety of forced-flow techniques, explaining how they markedly reduce developing time and result in less lateral diffusion and more compact spots
- Emphasizes the benefits of OPLC separation techniques, a method pioneered by the authors nearly forty years ago
- Discusses new developments, such as the BioArena system used to facilitate detection, isolation, and identification of new antimicrobials, antineoplastics, biopesticides, and other biologically active substances
1. Unique potentialities of layer liquid system
results, limitations, new demands Erno Tyihák 2. Overpressured-layer chromatography Erno Tyihák, Ágnes M. Móricz, and Emil Mincsovics 3. Centrifugal layer chromatography
Rotation planar chromatography Ágnes M. Móricz and Irena Vovk 4. Planar chromatography using electroosmotic flow Aneta Halka-Grysinska, Radoslaw L. Gwarda, Tadeusz H. Dzido 5. Layer dielectrochromatography
under external alternating current, LDEC Virginia Coman and Stefan Kreibik 6. Conventional and modern bioassays
detection, isolation, identification Ágnes M. Móricz and Péter G. Ott 7. BioArena system for studying key molecules as well as ingredients in biological samples Erno Tyihák and Ágnes M. Móricz 8. Determining progress directions in layer liquid chromatography
dreams and realities Erno Tyihák
Dr. Erno Tyihák's primary scientific achievements include the discovery of the formaldehyde cycle and formaldehydome system, the quadruple immune response of plants to pathogens, the unique role of endogenous formaldehyde and ozone in the antibiotic effect and the double effect of trace elements similar to trans-resveratrol. He was an early adopter of thin-layer chromatography methods and has been instrumental in moving the field forward, ultimately developing different patented versions of overpressured-layer chromatography, widely known as one of the most efficient separation techniques. Most recently he has invented the BioArena system, which integrates layer chromatographic separation with biological detection capabilities to explore in vivo phenomena such as the immunization of plants in greenhouse conditions. He holds PhD and DSc degrees from the Hungarian Academy of Sciences. He is Honorary Ass. Professor at the Semmelweis University, Budapest, Hungary, as well as Honorary Professor at the University of Szeged, Szeged, Hungary, and he was until recently a Scientific Advisor at the Plant Protection Institute, Hungarian Academy of Sciences Budapest, Hungary.