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G Protein-Coupled Receptors, Vol 132. Methods in Cell Biology

  • ID: 3504103
  • Book
  • 502 Pages
  • Elsevier Science and Technology
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G-Protein-Coupled Receptors: Signaling, Trafficking, and Regulation, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in G-Protein-Coupled Receptors, and includes sections on such topics signaling, trafficking and regulation.

  • Covers the increasingly appreciated cell biology field of G-protein-coupled receptors
  • Includes both established and new technologies
  • Contributed by experts in the field
  • Covers topics such as signaling, trafficking, and regulation

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Section 1 Trafficking, Localization and Imaging of GPCRs
1. Localization and Signaling of GPCRs in Lipid Rafts
Van Anthony M. Villar, Santiago G. Cuevas, Xiaoxu Zheng, Pedro A. Jose
2. Imaging GPCRs Trafficking and Signaling with Total Internal Reflection Fluorescence Microscopy in Cultured Neurons
Francheska Delgado-Peraza, Carlos Nogueras-Ortiz,  Agnes M. Acevedo Canabal, Cristina Roman-Vendrell, Guillermo A. Yudowski
3. Trafficking of Ciliary G-Protein Coupled Receptors
Jeremy McIntyre, Mellisa M. Hege, Nicolas F. Berbari
4. Single Molecule Resolution of G Protein-Coupled Receptor (GPCR) Complexes
Kim C Jonas, Ilpo Huhtaniemi, Aylin C Hanyaloglu
5. Quantification of the mRNA Expression of G-protein Coupled Receptors in Human Adipose Tissue
Stefan Amisten
Section 2 Signaling and Regulation of GPCRs
6. Studying the Regulation of Endosomal cAMP Production in GPCR Signaling
Alexandre Gidon, Timothy N. Feinstein, Kunhong Xiao, Jean-Pierre Vilardaga
7. GPCR-Radioligand Binding Assays
Colleen A. Flanagan
8. Assessing Smoothened-Mediated Hedgehog Signaling in Zebrafish
Teresa Casar Tena, Melanie Philipp
9. GPCRs and Actin-Cytoskeleton Dynamics
Genaro Vázquez-Victorio, Claudia González-Espinosa, Zyanya P. Espinosa-Riquer, Marina Macías-Silva
Section 3 Cellular Assays for GPCRs
10. Olfactory Receptor Signaling
Gabriela Antunes, Fabio Marques Simoes de Souza
11. Tracking GPCR Biosynthesis and Degradation Using a Non-Radioactive Pulse Chase Methodology
Richard B. Vallee Richard Wargachuk, Dominic Devost, Cynthia Zhou, Terence E. Hébert
12. Tango Assay for Ligand Induced GPCR
ß-arrestin2 Interaction: Application in Drug Discovery
Shalini Dogra, Chandan Sona, Ajeet Kumar, Prem N Yadav
13. Resonance Energy Transfer-Based Approaches to Study GPCRs 
Mohammed Akli Ayoub
14. Quantitative Analysis of G Protein-Coupled Receptor Internalization Using DnaE Intein-Based Assay
Bin Lu, Linjie Chen, Yaping Zhang, Ying Shi, Naiming Zhou
15. Cellular and Subcellular Context Determine Outputs from Signalling Biosensors
Dominic Devost, Nicolas Audet, Cynthia Zhou, Hiroyuki Kobayashi, Hélène Bonin, Viktorya Lukashova, Christian Le Gouill, Michel Bouvier, Terence E. Hébert
Section 4 Structural and Computational Investigation of GPCRs
16. Protease-Activated Receptors PARs in Cancer: Novel Biased Signaling and Targets for Therapy
Bar-Shavit R, Maoz M, Kancharla A, Jaber M, Agranovich D, Grisaru-Granovsky S, Uziely B
17. Computational Methods for Studying G Protein-Coupled Receptors (GPCRs)
Agnieszka A. Kaczor, Ewelina Rutkowska, Damian Bartuzi, Katarzyna M. Targowska-Duda, Dariusz Matosiuk, Jana Selent
18. Comparing Class A GPCRs to Bitter Taste Receptors: Structural Motifs, Ligand Interactions and Agonist-to-Antagonist Ratios
Antonella Di Pizio, Anat Levit, Michal Slutzki, Maik Behrens, Rafik Karaman, Masha Y Niv
19. What Can Simulations Tell Us About GPCRs: Integrating the Scales
Durba Sengupta, Manali Joshi, Chaitanya A. Athale, Amitabha Chattopadhyay
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