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Biomedical Electron Microscopy

  • ID: 3692635
  • Book
  • 548 Pages
  • Elsevier Science and Technology
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This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.

- Authored by the key leaders in the biological electron microscopy field- Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines- Introduces students on how to read and interpret electron micrographs

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Micrograph Interpretation Fixatives Fixative Vehicle Fixative Application Dehydration and Embedding Freezing and Freeze-Substitution Support Films Ultramicrotomy Section-Staining Microscopy Image Recording Photographic and Digital Printing Negative Staining Autoradiography Cytochemistry Immunocytochemistry Freeze-Fracturing and Shadowing Sampling and Quantitation Image Processing 3D Reconstructions Appendix: Practical Methods

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Maunsbach, Arvid B.
Afzelius, Björn A.
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