The Resolution Revolution: Recent Advances In cryoEM, Vol 579. Methods in Enzymology

  • ID: 3744815
  • Book
  • 486 Pages
  • Elsevier Science and Technology
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cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers research methods that determine the structures of biological molecules, a vital step for understanding their function
  • Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted
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  1. Direct Electron Detectors
    Methods in Enzymology (2016)
    Greg McMullan, Abdul R. Faruqi and Richard Henderson
  2. Specimen Behavior in the Electron Beam
    Robert M. Glaeser
  3. Specimen Preparation for High-Resolution Cryo-EM
    Christopher J. Russo and Lori A. Passmore
  4. Strategies for Automated Cryoem Data Collection Using Direct Detectors
    Anchi Cheng, Yong Zi Tan, Venkat Dandey, Clinton S. Potter and Bridget Carragher
  5. Processing of Cryo-EM Movie Data
    Zev A. Ripstein and John L. Rubinstein
  6. Processing of Structurally Heterogeneous Cryo-EM Data in RELION
    Sjors H.W. Scheres
  7. Single Particle Refinement and Variability Analysis in EMAN2.1
    Steven J. Ludtke
  8. Frealign: An Exploratory Tool for Single Particle Cryo-EM
    Nikolaus Grigorieff
  9. Testing the Validity of Single Particle Maps at Low and High Resolution
    Peter B. Rosenthal
  10. Tools for Model Building and Optimization into Near Atomic Resolution Electron Cryo-Microscopy Density Maps
    Frank DiMaio and Wah Chiu
  11. Refinement of Atomic Structures Against CryoEM Maps
    Garib N. Murshudov
  12. Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction
    Simon A. Fromm and Carsten Sachse
  13. Cryo-Electron Tomography and Subtomogram Averaging
    William Wan and John A.G. Briggs
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