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Cannabinoids and Their Receptors, Vol 593. Methods in Enzymology

  • ID: 4081017
  • Book
  • 560 Pages
  • Elsevier Science and Technology
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Cannabinoids and Their Receptors, Volume 593, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This updated volume includes comprehensive chapters on a variety of topics, including Real time cAMP signaling in response to CB1 activation, CB1 signaling in mitochondria, Lipidomics of cannabinoid systems, Studying endocannabinoid transport, Metabolic profiling of CB1 neutral antagonists, Approaches to assess biased signaling at the CB1 receptor, and the Development of CB1 allosteric modulators.

  • Continues the legacy of this premier serial with a new and updated release
  • Covers research cannabinoids and their receptors

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1. Mouse Neuroblastoma CB1 Cannabinoid Receptor-Stimulated [35S]GTP?S Binding: Total and Antibody-Targeted G? Protein-Specific Scintillation Proximity Assays 2. Protocols and Good Operating Practices in the Study of Cannabinoid Receptors 3. Real-Time Measurement of Cannabinoid Receptor-Mediated cAMP Signaling 4. Techniques for the Cellular and Subcellular Localization of Endocannabinoid Receptors and Enzymes in the Mammalian Brain 5. Endocannabinoid Transport Proteins: Discovery of Tools to Study Sterol Carrier Protein-2 6. Lipidomics: A Corrective Lens for Enzyme Myopia 7. Functional Analysis of Mitochondrial CB1 Cannabinoid Receptors (mtCB1) in the Brain 8. Modeling Neurodegenerative Disorders for Developing Cannabinoid-Based Neuroprotective Therapies 9. Metabolic Profiling of CB1 Neutral Antagonists 10. Ligand-Assisted Protein Structure (LAPS): An Experimental Paradigm for Characterizing Cannabinoid-Receptor Ligand-Binding Domains 11. New Methods for the Synthesis of Cannabidiol Derivatives 12. Approaches to Assess Biased Signaling at the CB1R Receptor 13. Design and Synthesis of Cannabinoid 1 Receptor (CB1R) Allosteric Modulators: Drug Discovery Applications 14. Assessing Allosteric Modulation of CB1 at the Receptor and Cellular Levels 15. Purification of Functional CB1 and Analysis by Site-Directed Fluorescence Labeling Methods 16. Mass Spectrometry Analysis of Human CB2 Cannabinoid Receptor and Its Associated Proteins 17. Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II 18. Methods for the Development of In Silico GPCR Models 19. Molecular Dynamics Methodologies for Probing Cannabinoid Ligand/Receptor Interaction

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