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Laboratory Methods in Microfluidics

  • ID: 4081068
  • Book
  • 178 Pages
  • Elsevier Science and Technology
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Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understand microfluidic technology applications. Microfluidics deals with the manipulation of small volumes of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering and environmental science.

The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay have made them attractive candidates to replace traditional experimental approaches.

Practical for students, instructors, and researchers, this book provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research.

  • Provides a number of detailed methods and instructions for experiments in microfluidics
  • Features an appendix that highlights several standard laboratory techniques, including reagent preparation plus a list of materials vendors for quick reference
  • Authored by a microfluidics expert with nearly a decade of research on the subject

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Introduction of microfluidics Experiment 1. Fabrication of a glass microfluidic device Experiment 2. Fabrication of paper microfluidic device for blood plasma separation Experiment 3. Fabrication and testing of PDMS microchip Experiment 4. Electrophoretic separation in a microchannel Experiment 5. Determination of electroosmotic flow in a straight glass microchannel Experiment 6. A simple experiment for the study of droplet microfluidics Experiment 7. Laminar flow and diffusion in microchannel Experiment 8. Beer's law using a smartphone Experiment 9. Acid-base titrations in paper analytical device Experiment 10. Simultaneous determination of glucose and protein in urine sample using paper microfluidics Experiment 11. Quantitative determination of total amino acids using paper microfluidics and a smartphone Experiment 12. Determination of nitrite ions using paper analytical device Experiment 13. Colorimetric determination of multiple metal ions on uPAD Experiment 14. Analysis of a mixture of paracetamol and 4-aminophenol in a paper-based microfluidic device Experiment 15. Synthesis of gold nanoparticles on microchip Experiment 16. Flow synthesis of organic dye on microchip Experiment 17. Enzyme-linked immunosorbent assay on a glass microfluidic device Experiment 18. Probing single cell mechanics on a microfluidic platform Experiment 19. Microfluidic device for observing algae phototaxis Experiment 20. A lab-on-a-chip experiment for protein immobilization

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Giri, Basant
Earned his doctorate in Analytical Chemistry from the University of Wyoming in 2013, where he developed microfluidic ELISA methods as part of his dissertation. Dr. Giri has been conducting research and instructing in the field of microfluidics for the past six years. He is currently a research scientist at Kathmandu Institute of Applied Sciences in Kathmandu, Nepal. His research interests involve developing microfluidics methods for various applications especially suitable for low resource settings
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