The Handbook of Histopathological Practices in Aquatic Environments: Guide to Histology for Environmental Toxicology offers readers in aquatic biology and other water-based environmental sciences a comprehensive resource on histopathology, which is a key tool in the growing field of ecotoxicology. This work brings together the necessary knowledge, from sample preparation, to trait identification, and scoring and data treatment. Furthermore, with examples from several groups of organisms (from worms to fish), these practices can be applied across a wide array of aquatic ecosystems. This book provides a step-by-step approach to solving the questions researchers encounter in aquatic biology and related fields.
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1. Introduction 2. General concepts 3. Sample preparation 4. Staining protocols 5. Microphotography 6. Qualitative, semi-quantitative and quantitative histopathology 7. Recipes 8. Troubleshooting
Dr Costa is a Marine Biologist specializing in Environmental Toxicology, with over 2 decades of experience in histology and histopathology, with emphasis on marine animals, both vertebrates and invertebrates. His expertise as a histologist ranges between mollusks and crustaceans to fish and known model organisms such as the zebrafish and murids. Whether using them biomarker candidates in environmental monitoring or as phenotypic anchors in complex and multidisciplinary toxicological studies, he has been applying and developing many histopathological techniques. He additionally teaches these techniques to graduate and post-graduate students with diverse academic backgrounds.