High Throughput Flow Assay Trends

  • ID: 4307242
  • Report
  • Region: Global
  • 60 Pages
  • Select Biosciences
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The Median Capex Annual Budget for HT Flow Detection Instruments was $150K-$200K

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on high throughput flow cytometry (HT flow) screening carried out in January 2017.

The survey was initiated as part of the publisher's tracking of emerging life science marketplaces and to update the previous HT Flow Assay Trends report 2015.

The questionnaire was compiled to meet the needs, requirements and interests of the flow cytometry vendor community.

The objectives were to document the current implementation and use of HT flow assays in screening, and to understand future user requirements. The aim was to compile a reference document on HT flow assays, which could be compared directly relative to our previous 2015 report.

Equal emphasis was given to soliciting opinion from the large molecule, small molecule and immunoassay screening segments worldwide.

The survey looked at the following aspects of HT flow screening, as practiced today (2017) and in a few cases as predicted for the future (2019):

  • why respondents have enabled or why they are interested in HT flow
  • how respondents have achieved HT flow analysis to date and typical throughput realised with current instrument approach
  • will implementation of HT flow mainly displace or complement existing screening technologies
  • key diseases/therapeutic areas targeted with HT flow screening
  • target classes most interested in applying HT flow assays
  • broad applications areas most using or most considering using HT flow assays
  • key applications for HT flow in respondent’s research
  • main benefits of HT flow versus other techniques
  • plate processing time considered adequate for HT flow needs
  • preferred sample presentation/handling format for HT flow and the target sample (aspirate) volume
  • number of parameters to be multiplexed in cell-based HT flow assays
  • number of analytes to be multiplexed in bead-based HT flow assays
  • samples types to be analysed with HT flow
  • where cell markers of interest in HT flow screens are located
  • main cell labelling technique/source
  • type of endpoints of most interest in HT flow screens
  • intention to carry out any front end processing or sample preparation prior to HT flow
  • number of different primary screens and number of wells to be tested per screen for cell-based and bead-based HT flow assays
  • estimated cost per sample (microplate well) analysed using cell-based and bead-based HT flow assays
  • vendor that first comes to mind when thinking of flow cytometry in general and HT flow specifically
  • plans to purchase any instrumentation to enable HT flow assays
  • capex budget for HT flow detection instruments
  • reagents and consumables budget for HT flow assays
  • how respondents have deployed HT flow instrumentation into their screening environment/core facility
  • biggest limitations (obstacles) in using HT flow assays today
  • ratings of possible limitations of HT flow
  • biggest hurdles in software and data analysis for HT flow
  • level of agreement with statements about the status of HT flow assays
  • and any unmet needs in HT flow screening today.
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Executive Summary
Table of Contents
Survey Methodology
Respondent's Organisation & Response to Survey
Respondent's Geographic Origin
Respondent's Company or Organisational Origin
Respondent's Job Role
Why Respondents Have Enabled or Why They Are Interested in HT Flow
Current Use of HT Flow Assays in Screening
How Respondents Have Achieved HT Flow Analysis to Date
Typical Throughput Realised to Date With Current Approach to HT Flow
Will Implementing HT Flow Mainly Displace or Complement Existing Screening Technologies (1)
Will Implementing HT Flow Mainly Displace or Complement Existing Screening Technologies (2)
Therapeutic/Disease Areas Targeting/Using HT Flow Assays
Target Classes Respondents Are Most Interested in Applying HT Flow Assays
Where Respondents Use (or Plan Use of) HT Flow Assays
Summary of Survey Findings (1)
Key Application Areas of HT Flow in Respondent’s Research (1)
Key Application Areas of HT Flow in Respondent’s Research (2)
Main Benefits of HT Flow Versus Other Techniques
Plate Processing Time Adequate for Respondent’s HT Flow Needs
Requirements for HT Flow Screening Assays
Number of Parameters Respondents Want to Multiplex in Cell Based HT Flow Assays
Number of Analytes Respondents Want to Multiplex in Bead-Based HT Flow Assays
Sample Types Wanted to Analyse With HT Flow Assays (1)
Sample Types Wanted to Analyse With HT Flow Assays (2)
Where Cell Markers of Interest in HT Flow Screens Are Located
Main Cell Labelling Technique/Source Used for HT Flow Assays
Type of Endpoints of Most Interest in HT Flow Screens
Respondents Who Plan to Carry Out Sample Prep On Cells Assayed By HT Flow
Number of HT Flow Primary Cell-Based Screens (HTS) Per Year
Number of Wells Per HT Flow Cell-Based Primary Screens (HTS)
Number of HT Flow Primary Bead-Based Screens (HTS) Per Year
Number of Wells Per HT Flow Bead-Based Primary Screens (HTS)
Cost Paid Per Sample (Well) Analysed Using HT Flow Cell-Based Assays
Cost Paid Per Sample (Well) Analysed Using HT Flow Bead-Based Assays
Vendors That First Come to Mind (1)
Vendors That First Come to Mind (2)
Summary of Survey Findings (2)
Plans to Purchase HT Flow Screening Instruments (1)
Plans to Purchase HT Flow Screening Instruments (2)
Plans to Purchase HT Flow Screening Instruments (3)
Annual Capex Budget for HT Flow Detection Instruments
Units Purchased Per Annual Capex Budget for HT Flow Detection Instruments
HT Flow Detection Instruments Market Estimate
Reagents & Consumables Budget for HT Flow Assays
HT Flow Assays Reagents & Consumables Market Estimate
Current/Planned Deployment of HT Flow Instrumentation in Screening
Biggest Limitations (Obstacles) in Using HT Flow Assays Today
Possible Limitations of HT Flow Assays
Biggest Hurdles in Software & Data Analysis for HT Flow Assays
Level of Agreement With Statements About The Status of HT Flow Assays
Unmet Needs in HT Flow Assays Today
Summary of Survey Findings (3)

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