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Fluorescence Microscopy In Life Sciences

  • ID: 4451238
  • Book
  • Region: Global
  • 731 Pages
  • Bentham Science Publishers Ltd
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Learn About Physical and Chemical Mechanisms Giving Rise to the Phenomenon of Luminescence and Fluorescence in a Comprehensive Way

Fluorescence Microscopy in Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
 
Key learning points covered in the book include:

  • Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches
  • The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology
  • Fluorescent chemical compounds
  • Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques

Fluorescence Microscopy in Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.

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  1. Foreword
  2. Preface
  3. Dedication
  4. Acknowledgements
  5. Introduction
  6. Physical Fundamentals of Luminescence
  7. Dyes and Fluorochromes
  8. Fluorescent Labels
  9. Spectral Analysis
  10. Fluorescence Instrumental and Techniques
  11. General Fluorescence Reactions
  12. Fluorescent Metal Complexes and Ion Indicators
  13. Luminescent Transition Metal Probes
  14. Lipids
  15. Polysaccharides
  16. Nucleic Acids
  17. Proteins
  18. Enzymes
  19. Vital Probes 1. Cell Organelles
  20. Vital Probes 2. Cell Viability and Signaling
  21. Immunofluorescence and FISH
  22. Luminescent Solid-State Markers
  23. Non-linear Optics
  24. Super-Resolution Microscopy
  25. Subject Index
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  • Juan Carlos Stockert
  • Alfonso Blázquez-Castro
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