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Ribozymes. Principles, Methods, Applications. Edition No. 1

  • ID: 5178801
  • Book
  • March 2021
  • 840 Pages
  • John Wiley and Sons Ltd
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Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field  

Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid base catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject.  

Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. 

  • The first reference to tie together all aspects of the multi-faceted field of ribozymes 
  • Features more than 30 comprehensive chapters in two volumes 
  • Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes  
  • Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions 
  • Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics 

Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

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INTRODUCTION
The chemical principles of RNA catalysis

NATURALLY OCCURRING RIBOZYMES
Biological roles of self-cleaving ribozymes
Chemical mechanisms of the nucleolytic ribozymes
The glmS ribozyme and its multifunctional coenzyme glucosamine-6-phosphate
The lariat capping ribozyme
Self-splicing group II introns
The Spliceosome: an RNA-Protein ribozyme derived from ancient mobile genetic elements
The ribosome and protein synthesis
The RNase P ribozyme
Ribozyme Discovery in Bacteria
Small self-cleaving ribozymes in the genomes of vertebrates

ENGINEERED RIBOZYMES
Phosphoryl transfer ribozymes
RNA replication and the RNA polymerase ribozyme
Maintenance of genetic information in the first ribocell
Ribozyme-catalyzed RNA recombination
Engineering of hairpin ribozymes for RNA processing reactions
Engineering of the Neurospora Varkud Satellite ribozyme for cleavage of non-natural stem-loop substrates
Chemical modifications in natural and engineered ribozymes
Ribozymes for regulation of gene expression
Development of flexizyme aminoacylation ribozymes and their applications
In vitro selected (deoxy)ribozymes that catalyze carbon-carbon-bond formation
Nucleic acid catalyzed RNA ligation and labeling

DNAZYMES
The chemical repertoire of DNA enzymes
Light-utilizing DNAzymes
Applications of DNAzymes in computing and nanotechnology.

RIBOZYMES AS TOOLS IN DIAGNOSTICS AND THERAPY
Optimization of antiviral ribozymes
DNAzymes as Biosensors
Compartmentalization-based technologies for in vitro selection and evolution of ribozymes and light-up RNA aptamers

TOOLS AND METHODS TO STUDY RIBOZYMES
Elucidation of ribozyme mechanisms at the example of the Pistol ribozyme
Strategies for crystallization of natural ribozymes
NMR spectroscopic investigation of ribozymes
Studying Ribozymes with Electron Paramagnetic Resonance Spectroscopy
Computational modeling methods for 3D structure prediction of ribozymes
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Sabine Müller
Benoît Masquida
Wade Winkler
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