The new ICRF Handbook of Genome Analysis is the essential guide to the enormous range of techniques available to the researcher for both the genetic and physical mapping of the genome, as well as the sequencing and analysis of DNA. It is both a protocol manual and a comprehensive information resource. Written by international experts, each chapter presents a state-of-the-art review of a methodology. Methods are fully described and evaluated; their advantages and disadvantages discussed; and their suitability for different investigations considered. Step-by-step protocols, including computer analyses, are given for 123 essential experimental procedures. 'Troubleshooting' sections discuss possible reasons for failure and offer remedies. The primary focus is on human genetics and the benefits of an understanding of the genome for the diagnosis and treatment of human disease. The book also considers the current state of progress in the analysis of genomes of many model organisms, including plants. A major part of the work provides detail on Internet resources as well as basic data on human and other genomes, including mapped disease genes and mouse knockouts.
- Covers not only the human genome in relation to cancers and other human diseases, but also the genomes of all important model organisms
- Contains 123 easy-to-follow protocols for essential experimental procedures
- Reviews a vast range of other information resources, including journals and the Internet
provides an invaluable listing of suppliers of laboratory materials
- Has been written by international experts from their own practical experience
- Is mandated by the Imperial Cancer Research Fund - a leader in research in this field
- Has a sturdy spiral binding within a hardback case for ease of use in the lab
Constructing And Using Genetic Maps.
Automated Map Construction.
Part II: Physical Mapping I: Cytogenetic Analysis.
Solid Tumor Cytogenetics.
In Situ Hybridisation.
Chromosome Sorting And Analysis.
Part III: Physical Mapping II: Long-Range Mapping And Gene Isolation.
Somatic Cell Hybrid Approaches To Genome Mapping.
Long-Range Physical Mapping.
Integrating Genetic And Physical Maps.
Strategies For Rapid Isolation Of Genes From Genomic DNA:Cdna Techniques Screening Cdna Libraries By Transient Expression In Mammalian Cells.
Part IV: Sequencing And Sequence Analysis.
Template Amplification And Purification.
Sequence Labelling And Detection.
Part V: Other Model Systems.
Somatic Gene Therapy.
Problems In Plant Genome Analysis.
Part VI: Internet Resources.
Internet Resources And How To Use Them.
Unix Survival Kit.
Databases And Genome Resource Centers.
Materials, Media And Solutions.
Preparation Of Blood Bottles And Processing Of Blood Samples.
Useful Addresses And Internet Connections.
Basic Data On Human And Other Genomes.
Catalogue Of Mapped Human Disease Genes.
Mouse Gene Knock-Out Tables.
Chromosome Aberrations Associated With Cancer.
S. Bryant ICRF Human Genetic Resources, ClareHall Laboratories, South Mimms.
Bryan Young Department of Molecular Oncology, St Bartholemew's Hospital.