PIER LUIGI LUISI
Università degli Studi di Roma Tre, Rome, ItalyCRISTIANO CHIARABELLIUniversità degli Studi di Roma Tre, Rome, Italy
Chemistry plays an important role in the emerging field of synthetic biology, providing the technologies that enable much of the research. In particular, chemical synthetic biology is concerned with the synthesis of new biological macromolecular structures (proteins and nucleic acids) and minimal life forms (semi–synthetic minimal cells) not found in nature, and – in contrast to other aspects of synthetic biology – without the use of genetic manipulation.
With contributions from leading internationals experts in the field, Chemical Synthetic Biology shows how chemistry underpins synthetic biology. Topics covered include:
- what roles do chemical tools perform in synthetic biology?
- synthetic nucleic acids, including never born RNAs and peptide nucleic acids (PNAs)
- searching forde novo totally random amino acid sequences
- random–sequence proteins consisting of five kinds of primitive amino acids
- experimental approaches for early evolution of protein function
- synthetic genetic codes as the basis of synthetic life
- towards safe genetically modified organisms through the chemical diversification of nucleic acids
- the minimal ribosome
- semi–synthetic minimal living cells
- replicators: components for systems chemistry
- biosafety, biosecurity, IPR and ethical challenges of chemical synthetic biology
Chemical Synthetic Biology is an essential guide to this fascinating new field, and will find a place on the bookshelves of researchers and students working in synthetic chemistry, synthetic and molecular biology, bioengineering, systems biology, computational genomics and bioinformatics.
Introduction (Pier Luigi Luisi).
Part One: Nucleic Acids.
1 Searching for Nucleic Acid Alternatives (Albert Eschenmoser).
2 Never–Born RNAs: Versatile Modules for Chemical Synthetic Biology (Davide De Lucrezia, Fabrizio Anella, Cristiano Chiarabelli, and Pier Luigi Luisi).
3 Synthetic Biology, Tinkering Biology, and Artificial Biology: A Perspective from Chemistry (Steven A. Benner, Fei Chen, and Zunyi Yang).
4 Peptide Nucleic Acids (PNAs) as a Tool in Chemical Biology (Peter E. Nielsen).
Part Two: Peptides and Proteins.
5 High Solubility of Random–Sequence Proteins Consisting of Five Kinds of Primitive Amino Acids (Nobuhide Doi, Koichi Kakukawa, Yuko Oishi, and Hiroshi Yanagawa).
6 Experimental Approach for Early Evolution of Protein Function (Hitoshi Toyota, Yuuki Hayashi, Asao Yamauchi, Takuyo Aita, and Tetsuya Yomo).
7 Searching for de novo Totally Random Amino Acid Sequences (Cristiano Chiarabelli, Cecilia Portela Pallares, and Anna Quintarelli).
Part Three: Complex Systems.
8 Synthetic Genetic Codes as the Basis of Synthetic Life (J. Tze–Fei Wong and Hong Xue).
9 Toward Safe Genetically Modified Organisms through the Chemical Diversification of Nucleic Acids (Piet Herdewijn and Philippe Marliere).
10 The Minimal Ribosome (Hiroshi Yamamoto, Markus Pech, Daniela Wittek, Isabella Moll, and Knud H. Nierhaus).
11 Semi–Synthetic Minimal Living Cells (Pasquale Stano, Francesca Ferri, and Pier Luigi Luisi).
Part Four: General Problems.
12 Replicators: Components for Systems Chemistry (Olga Taran and Günter von Kiedrowski).
13 Dealing with the Outer Reaches of Synthetic Biology Biosafety, Biosecurity, IPR, and Ethical Challenges of Chemical Synthetic Biology (Markus Schmidt, Malcolm Dando, and Anna Deplazes).
14 The Synthetic Approach in Biology: Epistemological Notes for Synthetic Biology (Pier Luigi Luisi).