Introduction to Nuclear Mechanics and Genome Regulation

Table of Contents

1. Physico-chemical constraints in DNA
2. 3D organization of chromosomes and functional gene clusters
3. Spatial dimension to DNA transcription, replication, damage and repair
4. Dynamics within the nucleus and functional microrheology
5. Cytoskeleton to nuclear links and prestressed nuclear architecture
6. Nuclear mechanotransduction and genetic networks
7. Chromatin plasticity during differentiation, development and reprogramming
8. Mechanical homeostasis of the nucleus during cell division and migration
9. Nuclear mechanics in diseases and as diagnostic markers
10. Evolutionary constraints in DNA packing and genome regulation

Authors

Shivashankar, G.V. Dr. G. V. Shivashankar is currently the Deputy Director of Mechanobiology Institute, National University of Singapore. Shivashankar's laboratory is focused on understanding the role of cell geometry on nuclear mechanics and genome regulation in living cells using a multi-disciplinary approach. He carried out his PhD research at the Rockefeller University (1994-1999) and Postdoctoral research at NEC Research Institute, Princeton, USA (1999-2000). He started his laboratory at the National Center for Biological Sciences, TIFR- Bangalore, India (2000-2009) before relocating to a tenured faculty position at the National University of Singapore in 2009. His scientific awards include the Birla Science Prize (2006) and the Swarnajayanthi Fellowship (2007), and he was elected to the Indian Academy of Sciences (2010). He also edited a Methods in Cell Biology volume on Nuclear Mechanics and Genome Regulation (2010), published by Elsevier. More recently, he headed the Joint Research Laboratory with the FIRC Institute of Molecular Oncology (IFOM), Milan, Italy and was appointed as an IFOM-NUS Chair Professor in 2014.