Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Vol 600. Methods in Enzymology

  • ID: 4335193
  • Book
  • 610 Pages
  • Elsevier Science and Technology
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Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Volume 600, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts.

  • Contributions by the leading experts in the field of DNA repair, recombination, replication and genome stability
  • Documents cutting edge methods

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Processing of DNA Double-Strand Breaks in Yeast

Robert Gnügge, Julyun Oh and Lorraine S. Symington

2. Methods to Study DNA End Resection I: Recombinant Protein Purification

Roopesh Anand, Cosimo Pinto and Petr Cejka

3. Methods to Study DNA End Resection II: Biochemical Reconstitution Assays

Cosimo Pinto, Roopesh Anand and Petr Cejka

4. Direct Quantitative Monitoring of Homology-Directed DNA Repair of Damaged Telomeres

Priyanka Verma, Robert L. Dilley, Melina T. Gyparaki and Roger A. Greenberg

5. Kinetic Analysis of the Exonuclease Activity of the Bacteriophage T4 Mre11-Rad50 Complex

Tibebe A. Teklemariam, Osvaldo D. Rivera and Scott W. Nelson

6. Observation and Analysis of RAD51 Nucleation Dynamics at Single-Monomer Resolution

Shyamal Subramanyam, Colin D. Kinz-Thompson, Ruben L. Gonzalez and Maria Spies

7. Determining the RAD51-DNA Nucleoprotein Filament Structure and Function By Cryo-Electron Microscopy

Lingyun Zhao, Jingfei Xu, Weixing Zhao, Patrick Sung, and Hong-Wei Wang

8. Expression, Purification, and Biochemical Evaluation of Human RAD51 Protein

Shyamal Subramanyam and Maria Spies

9. TIRF-Based Single-Molecule Detection of the RecA Presynaptic Filament Dynamics

Sung H. Kim

10. The RadA Recombinase and Paralogs of the Hyperthermophilic Archaeon Sulfolobus Solfataricus

Michael L. Rolfsmeier and Cynthia A. Haseltine

11. Reconstituting the 4-Strand DNA Strand Exchange

Olga M. Mazina and Alexander V. Mazin

12. Purification Of Saccharomyces Cerevisiae Homologous Recombination Proteins Dmc1 and Rdh54/Tid1 and a Fluorescent D-Loop Assay

Yuen-Ling Chan and Douglas K. Bishop

13. Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRET

Yupeng Qiu, Hye R. Koh and Sua Myong

14. The Recombination Mediator BRCA2: Architectural Plasticity of Recombination Intermediates Revealed by Single-Molecule Imaging (SFM/TIRF)

Arshdeep Sidhu, Dejan Ristic, Humberto Sánchez and Claire Wyman

15. Single-Molecule Dynamics and Localization of DNA Repair Proteins in Cells

Maarten W. Paul, Alex N. Zelensky, Claire Wyman and Roland Kanaar

16. Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy

Luisina De Tullio, Kyle Kaniecki and Eric C. Greene

17. Single-Molecule Analysis of Replication Protein A-DNA Interactions

Fletcher E. Bain, Laura A. Fischer, Ran Chen and Marc S. Wold

18. Single-Molecule Studies of ssDNA-Binding Proteins Exchange

Olivia Yang and Taekjip Ha

19. Dissecting the Recombination Mediator Activity of BRCA2 Using Biochemical Methods

Catharina von Nicolai, Åsa Ehlén, Juan S. Martinez and Aura Carreira

20. Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis

Qingwen Zhou, William K. Holloman and Milorad Kojic

21. GEN1 Endonuclease: Purification and Nuclease Assays

Ying Wai Chan and Stephen C. West

22. Biochemical and Structural Properties of Fungal Holliday Junction-Resolving Enzymes

Yijin Liu, Alasdair Freeman, Anne-Cécile Déclais, Anton Gartner and David M. J. Lilley

23. Preparation and Resolution of Holliday Junction DNA Recombination Intermediates

Rajvee Shah Punatar and Stephen C. West
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