Organs and Mini-Organs combines contributions from leading practitioners who work under the editorial control of an acclaimed researcher who also served for eight years as Editor-in-Chief of the journal Organogenesis, the first journal on this topic. The book begins with an introduction, but then delves into chapters that present advice on how to make organoids for many systems. In addition, case studies that illustrate the uses of organioids are presented, along with discussions on future directions and specific problems that need to be solved.
- Collects the best protocols of organoid cultures from diverse tissues
- Covers a wide range of organs
- Includes troubleshooting cases for common, but specific problems for each culture conditions
- Provides an entire section on the application of organoids
Section 2: Construction (each chapter reviews the technology and ends with a 'recipe') 2. Neurospheres (Michael Geusz
has the circadian rhythm story) 3. Cerebral cortex (Madeline Lancaster) 4. Midbrain (Karl-Heinz Krause) 5. Optic cup (Yoshiki Sasai) 6. Inner ear (Eri Hashino) 7. Gut (Owen Sansom, Glasgow) 8. Liver (Yo-ichi Tagawa) 9. Lung (Jason Spence) 10. Mammary gland (Charles Streuli or Andrew Ewald) 11. Prostate (Gail Prins or Michael Shen) 12. Immuno (Ankur Singh) 13. Thymus (Clare Blackburn) 14. Ovary (Norah Spears) 15. Kidney (Jamie Davies)
Section 3: Application 16. Physiology/ Pharmacology 17. Use of multiple organoids to represent multi-organ systems (Uwe Marx? - the liver/ neuropshere story) 18. Infection 19. Stomach organoids to study Helicobacter (Richard M Peek) 20. Gut organoids to study Salmonella (Jun Sun) 21. Cancer 22. Recreating tumours by modifying genes in organoids (Toshiro Sato) 23. High-throughput techniques for drug screening
Section 4: conclusion 24. Opportunities and likely future developments (Jamie Davies)
Since 1995 Davies has run his own laboratory at the University of Edinburgh, with a multidisciplinary focus on discovering how mammalian organs construct themselves and how we can use apply knowledge to build new tissues and organs for those in need. Some of the work of his 20-strong research team is 'conventional' developmental biology; identifying signals and mechanisms used in natural organ development. Some is bioinformatic analysis (we host the editorial office of an international database for renal development - www.gudmap.org - funded by the USA National Institutes of Health, and the www.guidetopharmacology.org database, an international effort for the International Union of Basic and Clinical Pharmacology). Some of his work is in tissue engineering - his lab has recently developed a method to produce engineered 'fetal kidneys' from simple suspensions of stem cells, an activity that attracted considerable press attention last year. Finally, his lab is pioneering the application of synthetic biology techniques to tissue engineering, to 'program' cells to make structures that are designed rather than evolved.
Davies has published around 140 research papers in the field of mammalian development, has published one major specialist monograph (Mechanisms of Morphogenesis, Elsevier, 2005 2nd Ed 2014), one public engagement book (Life Unfoloding, OUP, 2013 (Hardback), 2015 (paperback), now in translation also) and has edited three multi-author books in the fields of development, stem cells and tissue engineering. His contributions to research and teaching in this area have been recognized by having been elected a Fellow of the Royal Society of Biology, a Fellow of the Royal Society of Medicine and a Principal Fellow of the Higher Education Academy. Davies served as Deputy Chair of the National Centre for 3Rs, a government agency that promotes research that refines, reduces or replaces animal experiments. He has also served as Editor-in-Chief of the research journal Organogenesis for 8 years, and is currently an Editor of Journal of Anatomy and PLOS One.Lawrence, Melanie
Dr Lawrence has been working in the field of organoids for more than 4 years at the University of Edinburgh. She has a keen interest in exploring the functional aspects of organoids, and how this affects their application to in vitro toxicology studies. Lawrence has published research papers in the fields of renal development and organoids, and has co-authored book chapters relating to renal organoids. She has acted as a peer-reviewer for PLOS One and is a regular contributor to a scientific blog (kidneydevelopment.org). In addition, Lawrence is involved in community outreach programs aimed at secondary school children, to inspire the next generation of scientists and to encourage women to consider careers in STEM subjects.