The Aptamers Ligands: Powerful Tools from Molecular Evolution discusses these short, single strand oligonucleotides that can fold in a three-dimensional conformation and how they can provide a precise molecular recognition of a given target. These nucleic acid based ligands are identified from large naive combinatorial oligonucleotide libraries using a well-established technology called SELEX (systematic evolution of ligands by exponential enrichment). Aptamers can be extremely specific towards their target molecule with high selectivity and proper affinity dissociation constants. Moreover, they can be selected to recognize a high diversity of molecules, from very small compounds, to large macromolecules, such as proteins.
- Provides the molecular evolution of aptamers
- Includes a technological history on the subject
- Presents analytic and purification applications
Table of Contents
1. Concepts of evolution and molecular evolution2. Definitions and history of aptamers
3. The SELEX principles and its variations
4. How to analyze a SELEX
5. The chemical optimization of aptamers
6. Biosensors and other analytical applications
7. The aptamoaffinity
8. Applications for in vivo imaging
9. Ribozymes or natural aptamers
10. Drug delivery applications
11. Aptamer as direct therapeutic agent
