+353-1-416-8900REST OF WORLD
+44-20-3973-8888REST OF WORLD
1-917-300-0470EAST COAST U.S
1-800-526-8630U.S. (TOLL FREE)


Human Pluripotent Stem Cells. A Practical Guide. Edition No. 1

  • ID: 4411052
  • Book
  • January 2018
  • 176 Pages
  • John Wiley and Sons Ltd

Comprehensive coverage of the entire induced pluripotent stem cell basic work flow 

Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.

Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.

  • Includes helpful tips and tricks that are normally omitted from regular research papers
  • Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations
  • Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner
  • Written by experts with extensive experience in stem cell technologies

Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

Note: Product cover images may vary from those shown

1 Introduction 1

1.1 Biosafety 1

1.2 Biosafety Levels 2

1.3 Aseptic Technique 2

1.4 Storage 6

1.5 Contamination 7

1.6 Pluripotent Stem Cells 8

1.7 Procedures 9

References 16

2 Pluripotent Stem Cell Culture 17

2.1 Introduction 17

2.2 Materials 18

2.3 Solutions 19

2.4 Methods 21

References 47

3 Reprogramming 49

3.1 Introduction 49

3.2 Materials 49

3.3 Solutions 54

3.4 Methods 57

References 97

4 Characterization 99

4.1 Introduction 99

4.2 Materials 101

4.3 Solutions 103

4.4 Methods 104

References 128

5 Differentiation 131

5.1 Introduction 131

5.2 Materials 131

5.3 Solutions 133

5.4 Methods 135

References 151

Index 153

Note: Product cover images may vary from those shown
Chad C. MacArthur
Uma Lakshmipathy
Mahalakshmi Sridharan
Rene H. Quintanilla
Note: Product cover images may vary from those shown