Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems.
This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems.
This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances.
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Table of Contents
Part 1: Introduction 1. Microfluidic Cell Culture Technologies 2. Inertial microfluidics for stem cell isolation 3. Microfluidic Structures for Controlling Stem Cell Microenvironments 4. Microfluidic cell culture platforms with embedded nanoscale features 5. Microfluidic Vascular Networks for Engineered Tissues 6. Direct Write Assembly of Organ Models 7. 3D Bioprinting for Organ Models
Part II: Organ-Specific Disease and Drug Safety Models 8. Microfabricated Kidney Tissue Models 9. Microfluidic Hepatotoxicity Platform 10. Dynamic Gastrointestinal and Pulmonary Organ Models 11. Cardiac Models 12. Neural Tissue Microphysiological Systems 13. Models of the Female Reproductive Tract 14. Microscale Models of Inflammatory Diseases 15. Blood-Retinal Barrier-on-a -chip 16. Human blinking eye-on-a chip 17. Circulating tumor cell cultures for evaluation of anticancer treatment
Part III: Technologies for Sensing, Multiplexed and Interconnected Organ Models 18. Live Cell Analysis Under Shear Flow 19. High Throughput Flow Systems for Cardiovascular Research 20. Phaseguide technology for connected organ models 21. Electromagnetically microactuated platforms for dynamic microphysiological systems 22. Interconnected organ models for skin and liver 23. Heart-Liver-Vascular Interconnected Microphysiological Systems 24. Rocking Platform Technology for Interconnected Organ Models
