Marine enzymes and specialized metabolism - Part A, Volume 604 highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. These detailed protocols are written by experts who actively study and apply marine enzymes in biosynthesis and biotechnology. Comprehensive chapters in this latest release cover Chemoenzymatic synthesis of starting materials and characterization of halogenases requiring acyl carrier protein tethered substrates, Assaying biradical aryl coupling activity of CYP450 enzymes, the Characterization and application of marine microbial omega-3 polyunsaturated fatty acid synthesis, Catalase-related allene oxide synthase: on a biosynthetic route to fatty acid cyclopentenones, Haloalkane dehalogenases from marine microorganisms, and more.
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Section II: Biosynthesis by Bacterial Symbionts of Marine Invertebrate Animals 6. Chemoenzymatic Dissection of Polyketide ß-Branching in the Bryostatin Pathway Samuel T. Slocum, Andrew N. Lowell, Ashootosh N. Tripathi, Vikram V. Shende, Janet L. Smith and David H. Sherman 7. Radical S-Adenosylmethionine Peptide Epimerases: Detection of Activity and Characterization of d-Amino Acid Products Brandon I. Morinaka, Anna L. Vagstad and Jörn Piel 8. Cobalamin-Dependent C-Methyltransferases From Marine Microbes: Accessibility via Rhizobia Expression Michael F. Freeman 9. Biosynthetic Insights of Calyculin- and Misakinolide-Type Compounds in "Candidatus Entotheonella sp." Agustinus R. Uria, Jörn Piel and Toshiyuki Wakimoto
Section III: Natural Product Biosynthetic Enzymology of Marine Bacteria and Fungi 10. Chemoenzymatic Synthesis of Starting Materials and Characterization of Halogenases Requiring Acyl Carrier Protein-Tethered Substrates Hem R. Thapa, Andrew J. Lail, Neha Garg and Vinayak Agarwal 11. Preparation, Assay, and Application of Chlorinase SalL for the Chemoenzymatic Synthesis of S-Adenosyl-L-Methionine and Analogs Tony D. Davis, Sylvia Kunakom, Michael D. Burkart and Alessandra S. Eustaquio 12. In Vitro Analysis of Cyanobacterial Nonheme Iron-Dependent Aliphatic Halogenases WelO5 and AmbO5 Xinyu Liu 13. Characterization and Biochemical Assays of Streptomyces Vanadium-Dependent Chloroperoxidases Shaun M.K. McKinnie, Zachary D. Miles and Bradley S. Moore 14. Unusual "Head-to-Torso" Coupling of Terpene Precursors as a New Strategy for the Structural Diversification of Natural Products Robin Teufel 15. Identification of Enzymes Involved in Sesterterpene Biosynthesis in Marine Fungi Jingjing Yan, Jingjing Guo, Wei Yuan, Wanying Mai and Kui Hong 16. Assaying Oxidative Coupling Activity of CYP450 Enzymes Vinayak Agarwal 17. Preparation and Characterization of the Favorskiiase Flavoprotein EncM and Its Distinctive Flavin-N5-Oxide Cofactor Robin Teufel