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Aptamers for Analytical Applications. Affinity Acquisition and Method Design

  • ID: 4469840
  • Book
  • November 2018
  • 432 Pages
  • John Wiley and Sons Ltd
Adopting an analyst and developer–centered approach, this comprehensive monograph investigates in detail analytical method development and improvements with established systems and beyond.

Following an introduction and common protocols for aptamer synthesis, different optimization strategies to obtain higher affinities to target molecules are presented. Characterization of aptamer–target molecule interaction and immobilization strategies then form the foundation for an in–depth discussion of method developments for all relevant applications, from colorimetric methods to lateral flow immunoassays, each of which is treated in a separate chapter.

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Introduction of aptamers and its analytical merits

Protocols of SELEX and important SELEX variants

In chemico modification of aptamers for better recognition

In silico modification of aptamers for better recognition

Immobilization of aptamers on substrates

Characterization of aptamer–ligand complexes

Utilization of aptamers for sample preparation and pretreatment

Development of colorimetric aptamer–based analytical methods

Development of aptamer linked immunosorbent assays

Development of aptamer–based fluorescence methods

Development of aptamer–based electrochemical methods

Development of aptamer–based optical sensors

Development of aptamer–based lateral flow immunoassays

Challenges of SELEX and demerits of aptamer–based bioassays

Visions of aptamers and emerging applications
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Yiyang Dong
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