High-Density Sequencing Applications in Microbial Molecular Genetics, Vol 612. Methods in Enzymology

  • ID: 4579846
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymologyseries provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more.

Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics.

  • Detail methods used in research articles that were recently published in leading journals
  • Provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology
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- Characterizing the Role of Exoribonucleases in the Control of Microbial Gene Expression: Differential RNA-Seq

Vânia Pobre and Cecília M. Arraiano

- Conformational Studies of Bacterial Chromosomes by High-Throughput Sequencing Methods

Virginia S. Lioy and Frédéric Boccard

- Large-Scale Measurement of mRNA Degradation in Escherichia coli: To Delay or Not to Delay

Sandrine Laguerre, Ignacio González, Sébastien Nouaille, Annick Moisan, Nathalie Villa-Vialaneix, Christine Gaspin, Marie Bouvier, Agamemnon J. Carpousis, Muriel Cocaign-Bousquet and Laurence Girbal

- FASTBAC-Seq: Functional AnalysiS of Toxin-Antitoxin Systems in BACteria by Deep Sequencing

Sara Masachis, Nicolas J. Tourasse, Sandrine Chabas, Olivier Bouchez and Fabien Darfeuille

- The Challenges of Genome-Wide Studies in a Unicellular Eukaryote With Two Nuclear Genomes

Sandra Duharcourt and Linda Sperling

- CLIP-Seq in Bacteria: Global Recognition Patterns of Bacterial RNA-Binding Proteins

Liis Andresen and Erik Holmqvist

- High-Resolution Profiling of NMD Targets in Yeast

Feng He, Alper Celik, Richard Baker and Allan Jacobson

- Generation of a Metagenomics Proximity Ligation 3C Library of a Mammalian Gut Microbiota

Théo Foutel-Rodier, Agnès Thierry, Romain Koszul and Martial Marbouty

- Genome-Wide Mapping of Yeast Retrotransposon Integration Target Sites

Anastasia Barkova, Amna Asif-Laidin and Pascale Lesage

- Genome-Wide Quantitation of Protein Synthesis Rates in Bacteria

Grace E. Johnson and Gene-Wei Li

- On the Prowl: An In Vivo Method to Identify RNA Partners of a sRNA

Marie-Claude Carrier, Claire Morin and Eric Massé

- Analysis of Bacterial Transcription by "Massively Systematic Transcript End Readout," MASTER

Irina O. Vvedenskaya, Seth R. Goldman and Bryce E. Nickels

- Transcriptomic Approaches for Studying Quorum Sensing in Vibrio cholerae

Roman Herzog and Kai Papenfort

- In Vitro Study of the Major Bacillus subtilis Ribonucleases Y and J

Liliana Mora, Saravuth Ngo, Soumaya Laalami and Harald Putzer

- Mapping 5'-Ends and Their Phosphorylation State With EMOTE, TSS-EMOTE, and nEMOTE

Peter Redder

- MS2-Affinity Purification Coupled With RNA Sequencing Approach in the Human Pathogen Staphylococcus aureus

David Lalaouna, Emma Desgranges, Isabelle Caldelari and Stefano Marzi

- Noncoding RNAs in Archaea: Genome-Wide Identification and Functional Classification

Anne Buddeweg, Michael Daume, Lennart Randau and Ruth A. Schmitz

- Methodology for Ribosome Profiling of Key Stages of the Caulobacter crescentus Cell Cycle

James R. Aretakis, Nadra Al-Husini and Jared M. Schrader

- Transcriptome-Wide Analysis of Protein-RNA and RNA-RNA Interactions in Pathogenic Bacteria

Jai J. Tree, Kenn Gerdes and David Tollervey

- Profiling RNA Polymerase II Phosphorylation Genome-Wide in Fission Yeast

Tea Kecman, Dong-Hyuk Heo and Lidia Vasiljeva

- Toward Cell Type-Specific In Vivo Dual RNA-Seq

Lutz Frönicke, Denise N. Bronner, Mariana X. Byndloss, Bridget McLaughlin, Andreas J. Bäumler and Alexander J. Westermann

- Genomic Analysis of DNA Double-Strand Break Repair in Escherichia coli

A.M. Mahedi Hasan, Benura Azeroglu and David R.F. Leach
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