High-Density Sequencing Applications in Microbial Molecular Genetics, Vol 612. Methods in Enzymology

  • ID: 4579846
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymologyseries provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more.

Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics.

  • Detail methods used in research articles that were recently published in leading journals
  • Provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology
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1. Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq. Cecilia Arraiano 2. Conformational studies of bacterial chromosomes by high-throughput sequencing methods Frederic Boccard 3. Measuring mRNA degradation: delay or not delay Muriel Cocaign-Bousquet 4. Toxin-antitoxin systems in Helicobacter pylori Fabien Darfeuille 5. The challenges of genome-wide analyses in a eukaryotic microorganism with two nuclear genomes Sandra Duharcourt 6. Global recognition patterns of bacterial RNA-binding proteins Erik Holmqvist 7. High-resolution profiling of NMD targets Allan Jacobson 8. Generation of a metagenomic 3C/Hi-C library of human gut microbiota Romain Koszul 9. Genome-wide mapping of yeast retrotransposons integration target sites Pascale Lesage 10. Measuring protein synthesis rates Gene-Wei Li 11. Finding unsuspected partners of small RNAs with new screening approaches Eric Masse 12. Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output Bryce Nikels 13. RNA-based control of quorum sensing in Vibrio cholerae Kai Papenfort 14. The role of membrane tethering in the initiation of mRNA decay in Bacillus subtilis Harald Putzer 15. RNA 5' end mapping and phosphorylation state in Staphylococcus aureus Peter Redder 16. Identification of RNA targets of Staphylococcus aureus RNase III combining catalytically inactive mutant enzyme and RNA sequencing Pascale Romby 17. Non-coding RNAs in Archaea: What do we know today? Ruth Schmitz 18. Ribosome profiling of the Caulobacter cell cycle Jared Schrader 19. High-throughput analysis of protein-RNA and RNA-RNA interactions in pathogenic bacteria Jai Tree 20. Profiling RNA polymerase II modifications genome-wide in fission yeast Lidia Vasiljeva 21. Multi-organism RNA-seq approaches to bacterial infections Alexander J. Westermann 22. Sort-Seq in the analysis of genetic regulatory sequences Wade C. Winkler 23. Genomic analysis of DNA double-strand break repair in Escherichia coli David Leach
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