Biological NMR Part A, Vol 614. Methods in Enzymology

  • ID: 4593650
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Biological NMR, Part A, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Protein methyl labeling, Membrane protein expression - yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs and micelles MP host, PTM - phosphorylation, PTM - lipidation, Screening platform for receptor-ligand discovery

Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, Structure determination and computer analysis, and much more.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods of Enzymology series
  • Updated release includes the latest information on the Biological NMR
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Sample characterization/preparation 1. Protein methyl labeling 2. Membrane protein expression
yeast 3. Protein aromatic labeling 4. His-tag/Metal contamination 5. Bicelles, nanodiscs and micelles MP host 6. PTM
phosphorylation 7. PTM
lipidation 8. Screening platform for receptor-ligand discovery

Solution Spectroscopy 9. Large protein strategies 10. NUS data collection/analysis 11. F19 incl. hydration 12. ODNP
hydration 13. Reverse micelle
Hydration

Solid State Spectroscopy 14. SS NMR membrane proteins 15. SS NMR soluble/aggregate proteins 16. SS DNP
general 17. SS NMR nucleic acids

Structure determination and computer analysis 18. CS-Rosetta 19. Sparse NMR data for modeling protein structures 20. Nucleic acids 21. MD analysis

Exotica 22. In-cell DNP assisted solid state amyloid NMR 23. Reverse micelle encapsulation 24. High pressure NMR

Dynamics 25. Lysine/arginine dynamics 26. CPMG/T1rho 27. Fast dynamics/Entropy analysis

Metabolomics 28. SUMMIT MS/NMR

Screening 29. STD (ligand based) 30. WaterLoggsy 31. Dissolution DNP 32. Preparation and solution NMR spectroscopy of glycosylated proteins

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Wand, A.Joshua
Dr. A. Joshua Wand teaches at the Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, USA
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