Tools, Techniques and Protocols for Monitoring Environmental Contaminants describes information on the strategic integration of available monitoring methods with molecular techniques, with a focus on omics (DNA, RNA and protein based) and molecular imprinted polymer and nanomaterial based advanced biosensors for environmental applications. It discusses the most commonly practiced analytic techniques, such as HPLC, MS, GCMS and traditional biosensors, giving an overview of the benefits of advanced biosensors over commonly practiced methods in the rapid and reliable assessment of environmental contaminants.
As environmental contaminants have become one of the serious concerns in terms of their rapid growth and monitoring in the environment, which is often limited due to costly and laborious methods, this book provides a comprehensive update on their removal, the challenges they create for environmental regulatory agencies, and their diverse effects on terrestrial and aquatic environments.
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Overview of Environmental monitoring methodology 1. An overview of analytical methodology for environmental monitoring 2. A perspective of advanced biosensors for environmental monitoring 3. An overview of sensitivity and selectivity of biosensors 4. Potential application of advanced sensor systems for real time monitoring of waste water treatment plants
DNA and RNA and aptamer based techniques 5. An overview of DNA/RNA based monitoring tools and biosensors. Benefits and applications in environmental toxicology 6. Recent advances in oligonucleotide based sensor technology for detection of pharmaceuticals in the environment 7. Recent advances in oligonucleotide based sensor technology for detection of endocrine disrupting chemicals (EDC) in the environment 8. Oligonucleotide based biosensors for detection of environmental heavy metal contaminants
Protein/Metabolite based Techniques (DNAzymes, Aptazymes, Enzymes, Antibody etc.) 9. An overview of Protein/metabolite based advanced monitoring tools and biosensors. Benefits and applications in environmental toxicology 10. Advances in protein based biosensors for detection of pharmaceutical contaminants in the environment 11. Advances in protein based biosensors for detection of pesticide contaminants in the environment 12. Advances in protein based biosensors for detection of metal contaminants in the environment 13. Advances in whole cell based biosensors for environmental monitoring 14. Recent trends in advancement of antibody based biosensor systems for environmental monitoring 15. An overview of enzyme based biosensors for environmental monitoring
Synthetic polymer/other methods 16. An overview of advanced monitoring tools and sensors. Benefits and applications in environmental toxicology 17. Molecular imprinted polymer based biosensors for detection of pharmaceutical contaminants in the environment 18. Molecular imprinted polymer based biosensors for detection of pesticide contaminants in the environment 19. Molecular imprinted polymer based biosensors for detection of metal contaminants in the environment 20. Recent advances in nanomaterial based sensors as tool for environmental monitoring
Dr. S. K. Brar is Professor in Environmental Engineering. She holds Master's Degrees in Organic Chemistry and in Technology in Environmental Sciences and Engineering, and a Ph.D. in Environmental Biotechnology. Her research interests lie in bioprocesses and the valorization of residues (agricultural; agro-industrial; industrial and municipal) into high value-bioproducts, such as enzymes, organic acids, platform chemicals, biocontrol agents, biopesticides, butanol and biohydrogen. She is also interested in the fate of emerging contaminants during value-addition of wastewater and wastewater sludge in turn finding suitable biological detoxification technologies. In 2014, Dr. S. K. Brar was elected as a member of The College of New Scholars, Artists and Scientists of the Royal Society of Canada in recognition for outstanding performance in her field of environmental biotechnology. She has more than 300 research publications, including: five books, 56 chapters, 230 original research papers, 60 research communications in conferences and has registered 2 patents to her credit.
Krishnamoorthy Hegde FRQNT Researcher, Institut National de la Recherche Scientifique (Eau, Terre et Environnement, INRS-ETE), Quebec, Canada.
Krishnamoorthy Hegde. Krishnamoorthy Hegde is an FRQNT researcher at Institut National de la Recherche Scientifique (Eau, Terre et Environnement, INRS-ETE), Québec, Canada. His research interest lies in Green synthesis of functionalized polymers, Development of advanced biosensors for environmental applications, conversion of biomass waste and industrial waste for biofuel production, Technology development for treatment of environmental contaminants in effluents, waste water and drinking water, development for microbial and biocatalyst assisted degradation of petroleum crude wastes (bioremediation). He completed his PhD in Biotechnology from Indian Institute of Technology Guwahati in 2014 and holds more than 8 years' industrial and academic research experience in the field of molecular biology, Protein Chemistry, Genetic Engineering Technology, metabolic engineering, biocatalysis, Bioprocess Development, and renewable energy. During his industrial research tenure, he worked for nearly 3 years, as a project leader at his former work place (Navya Biologicals Pvt. Ltd., India) towards metabolic pathway engineering of yeast (Saccharomyces cerevisiae, Pichia pastoris) for production of therapeutic recombinant proteins. One of the upstream metabolic engineering project he was involved as a lead researcher in the year 2007-2008 is now at the finishing stage (formulation) to release its product to market in India. Further, during his industrial postdoctoral research in India, he contributed significantly at Hindustan Petroleum Corporation Limited, Green R&D (HPCL GRD), India towards sustainable biocatalyst development, development of novel technology for bioremediation and effluent waste treatment for petrochemical wastes, technology transfer and field trials of the 'in-house' techniques developed at laboratory scale in petroleum refineries. In addition, he also worked on industrial-academic collaborative projects towards pathway engineering and strain improvement for biobutanol production during his industrial postdoctoral research. His research contribution is credited with more than 25 peer reviewed publications and 5 patents.
Vinayak Laxman Pachapur Institut National de la Recherche Scientifique, (Eau, Terre et Environnement, INRS-ETE), Quebec, Canada.
Dr. Pachapur is currently a researcher at CRIQ (Research Center Industrial Du Québec) as industrial partner and Institut National de la Recherche Scientifique, (Eau, Terre et Environnement, INRS-ETE) as university partner under the receipt of "MITACS Accelerate Scholarship-2018". Dr. Pachapur also holds the "FRQNT Post-Doctoral Merit scholarship-2017," carried out at LAVAL University (Department of Civil Engineering and Water Engineering) on novel hydrolysis for forest residues. Dr. Pachapur completed his Ph.D in Environmental Biotechnology from INRS-ETE in 2017 on Bio-hydrogen production using co-culture system. During PhD tenure, he has achieved 10 article publications across international journals (8 research and 2 reviews) and 1 US and CANADA patent submission. Recently his Ph.D thesis was awarded as "BEST THESIS PRIZE FOR INOVATION-2017" across three INRS centers. At "National Biodiesel Conference" in San Diego, U.S.A in January 2017, his Ph.D report was recognized as "Next Generation Scientists" and presentation was awarded with a scholarship. Dr. Pachapur has nearly 8+ years of industrial and academic experience in the related field of molecular, microbiology, fermentation, analytical and proteomics platforms. Working on emerging areas of industrial biotechnology such as bioremediation using enzymes, bio-valorization of industry wastes, development of novel-green method, molecular profiling on microbial pathways, co-culture fermentation for biofuels and platform chemicals production from industrial wastes. Till now, Dr. Pachapur has achieved 25+ peer reviewed publications across internationals journals, book chapters with above 300 citations, 10 research communications in conferences and 2 patent submissions to his credit.