CRISPR-Cas Enzymes, Vol 616. Methods in Enzymology

  • ID: 4612920
  • Book
  • 452 Pages
  • Elsevier Science and Technology
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CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on the CRISPR-Cas Enzymes
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- Predicting and visualizing features of CRISPR-Cas systems

Matthew A. Nethery and Rodolphe Barrangou

- Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems

Yibei Xiao and Ailong Ke

- Sortase-mediated fluorescent labeling of CRISPR complexes

Kaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein

- Fluorescence-based methods for measuring target interference by CRISPR-Cas systems

Phong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital

- Probing Cascade complex composition and stability using native mass spectrometry techniques

Angela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner

- High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP

Joseph T. Wade

- Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance

Bartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau

- In vitro assembly of thermostable Csm complex in CRISPR-Cas type III/A system

Kwang-Hyun Park, Yan An and Eui-Jeon Woo

- Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems

Christophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White

- A pipeline for characterization of novel Cas9 orthologs

Tautvydas Karvelis, Joshua K. Young and Virginijus Siksnys

- Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells

Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek

- Directed evolution studies of a thermophilic Type II-C Cas9

Travis H. Hand, Anuska Das and Hong Li

- Kinetic characterization of Cas9 enzymes

Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W. Taylor and Kenneth A. Johnson

- Single-molecule FRET studies of Cas9 endonuclease

Viktorija Globyte and Chirlmin Joo

- CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA

Vladimir Mekler, Konstantin Kuznedelov, Leonid Minakhin, Karthik Murugan, Dipali G. Sashital and Konstantin Severinov

- Adapting dCas9-APEX2 for subnuclear proteomic profiling

Xin D. Gao, Tomás C. Rodríguez and Erik J. Sontheimer

- CRISPR-Cas10 assisted editing of virulent staphylococcal phages

S.M. Nayeemul Bari and Asma Hatoum-Aslan

- Reconstitution of CRISPR adaptation in vitro and its detection by PCR

Robert D. Fagerlund, Timothy J. Ferguson, Howard W.R. Maxwell, Helen K. Opel-Reading, Kurt L. Krause and Peter C. Fineran
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