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Cell-free Protein Expression Market - Growth, Trends, and Forecast (2019 - 2024)

  • ID: 4622545
  • Report
  • 118 pages
  • Mordor Intelligence
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  • Biotechrabbit GmbH
  • CellFree Sciences Co. Ltd
  • Cube Biotech GmbH
  • GeneCopoeia Inc.
  • Jena Bioscience GmbH
  • Promega Corporation
  • MORE
Market Overview

The major factors for the growth of the cell-free protein expression market include the increasing R&D in the fields of proteomics and genomics, high prevalence of cancer and infectious diseases, and shorter expression time and structural modification.

Cell-free protein expression employs cell lysates for the production of the desired recombinant proteins, with the primary advantage of this technique being the aversion of workflow hurdles of maintaining cell culture or living cells. Cell lysates are produced by breakdown and use of cellular components of eukaryotic or bacterial cells. Currently, several cell-free protein expression systems are commercially available, based on E. coli, rabbit reticulocytes, wheat germs, human cell lines, and insect cells. In vitro protein expression system has significant advantages over living cell-based protein expression, including a shorter duration of the process, isotopic labeling of proteins, incorporation of non-natural amino acids, and optimization of protein complexes. Pertaining to the recent trend of more research in personalized medicine and growing needs for biological therapies for the management of cancers, the ability to enable high throughput production is a major driver for the adoption of cell-free protein expression techniques by pharmaceutical and biotechnology firms.

Scope of the Report

As per the scope of this report, cell-free protein expression or in vitro protein synthesis is a process that involves the production of the recombinant proteins in cell lysate solution, without using cell culture or living cells. Cell-free protein expression can be carried out using different cell lysates, such as those of E. coli, rabbit reticulocytes, wheat germs, human cells, and insect cells.

Key Market Trends

E. Coli Lysate is the Segment by Lysate Systems is expected to be the Fastest Growing Segment Over the Forecast Period

E. coli lysates are the most commonly used systems among all available commercial lysate systems. E. coli lysates are devoid of endogenous genetic messages and have advantages, such as very high protein yield and tolerability to most additives. Furthermore, E. coli-based systems are being used in recombinant techniques for the manufacture of various biological therapeutics and insulin. Continuous use has enabled research on interactions and tolerability of by-products of E. coli-based systems, and significant present scientific literature enables its rapid adoption by industries and academics. In E. coli-based systems, codons used are different, with some being eukaryotic specific. Also, post-translational modifications are not possible, which in turn, limit the system’s usage in the synthesis of specific human therapeutics. However, significant advancements have been made in order to address these problems. Therefore, the E.coli lysate sub-segment is expected to register a steady growth rate during the forecast period, owing to high adoption rates and recent developments.

North America is Expected to Dominate the Market

North America is found to hold a major share for the cell-free protein expression market and is expected to show a similar trend over the forecast period, without significant fluctuations. The primary factors behind the large market size include the increasing prevalence of chronic diseases, such as cancer, aging population, growing demand for targeted and personalized medicine, the presence of major R&D facilities, and favorable government initiatives in the United States. There is a growing trend of cell-based research in the US academia and biotechnology industries. In recent years, several government initiatives have been launched supplementing the growth of the cell-free protein expression market in the United States. For instance, 2015 saw the introduction of the Precision Medicine Initiative in the United States, which was intended to revolutionize the treatment of many chronic diseases, including cancer. Initiatives like these are presenting a favorable environment for pharmaceutical and biotechnology firms to develop and market protein-based therapeutics, thus, fueling the growth of the cell-free protein expression market.

Competitive Landscape

The market studied is a moderately consolidated market owing to the presence of small and large market players. Some of the market players are biotechrabbit GmbH, CellFree Sciences Co. Ltd, Cube Biotech GmbH, GeneCopoeia Inc., Jena Bioscience GmbH, Promega Corporation, Qiagen NV, Sigma-Aldrich Corporation (Merck KGaA), Takara Bio Inc., and Thermo Fisher Scientific Inc.
Note: Product cover images may vary from those shown
2 of 4


  • Biotechrabbit GmbH
  • CellFree Sciences Co. Ltd
  • Cube Biotech GmbH
  • GeneCopoeia Inc.
  • Jena Bioscience GmbH
  • Promega Corporation
  • MORE
1.1 Study Deliverables
1.2 Study Assumptions
1.3 Scope of the Study



4.1 Market Overview
4.2 Market Drivers
4.2.1 Increasing R&D in the Fields of Proteomics and Genomics
4.2.2 High Prevalence of Cancer and Infectious Diseases
4.2.3 Shorter Expression Time and Structural Modification
4.3 Market Restraints
4.3.1 High Cost
4.3.2 Lack of Effective Systems for Post-translational Modifications
4.4 Porter's Five Force Analysis
4.4.1 Threat of New Entrants
4.4.2 Bargaining Power of Buyers/Consumers
4.4.3 Bargaining Power of Suppliers
4.4.4 Threat of Substitute Products
4.4.5 Intensity of Competitive Rivalry

5.1 By Products
5.1.1 Accessories and Consumables
5.1.2 Lysate Systems E. Coli Lysate Wheat Germ Extract Lysate Rabbit Reticulocyte Lysate Insect Cell Lysate Human Cell Lysate Other
5.2 By Application
5.2.1 Enzyme Engineering
5.2.2 High Throughput Production
5.2.3 Protein Labeling
5.2.4 Protein-Protein Interaction
5.2.5 Other
5.3 Geography
5.3.1 North America US Canada Mexico
5.3.2 Europe Germany UK France Italy Spain Rest of Europe
5.3.3 Asia Pacific China Japan India Australia South Korea Rest of Asia-Pacific
5.3.4 Middle East and Africa GCC South Africa Rest of Middle East and Africa
5.3.5 South America Brazil Argentina Rest of South America

6.1 Company Profiles
6.1.1 Biotechrabbit GmbH
6.1.2 CellFree Sciences Co. Ltd
6.1.3 Cube Biotech GmbH
6.1.4 GeneCopoeia Inc.
6.1.5 Jena Bioscience GmbH
6.1.6 Promega Corporation
6.1.7 Qiagen NV
6.1.8 Sigma-Aldrich Corporation (Merck KGaA)
6.1.9 Takara Bio Inc.
6.1.10 Thermo Fisher Scientific Inc.

Note: Product cover images may vary from those shown
3 of 4


4 of 4
  • Biotechrabbit GmbH
  • CellFree Sciences Co. Ltd
  • Cube Biotech GmbH
  • GeneCopoeia Inc.
  • Jena Bioscience GmbH
  • Promega Corporation
  • Qiagen NV
  • Sigma-Aldrich Corporation (Merck KGaA)
  • Takara Bio Inc.
  • Thermo Fisher Scientific Inc.
Note: Product cover images may vary from those shown